PUBLICATION
Microstructured Surface Arrays for Injection of Zebrafish Larvae
- Authors
- Ellett, F., Irimia, D.
- ID
- ZDB-PUB-170204-1
- Date
- 2017
- Source
- Zebrafish 14(2): 140-145 (Journal)
- Registered Authors
- Ellett, Felix
- Keywords
- infection, larvae, microinjection, microstructures, xenotransplantation
- MeSH Terms
-
- Animals
- Laboratory Animal Science
- Larva
- Microinjections/methods
- Microinjections/veterinary*
- Silicones
- Zebrafish/anatomy & histology*
- PubMed
- 28151697 Full text @ Zebrafish
Citation
Ellett, F., Irimia, D. (2017) Microstructured Surface Arrays for Injection of Zebrafish Larvae. Zebrafish. 14(2):140-145.
Abstract
Microinjection of zebrafish larvae is an essential technique for delivery of treatments, dyes, microbes, and xenotransplantation into various tissues. Although a number of casts are available to orient embryos at the single-cell stage, no device has been specifically designed to position hatching-stage larvae for microinjection of different tissues. In this study, we present a reusable silicone device consisting of arrayed microstructures, designed to immobilize 2 days postfertilization larvae in lateral, ventral, and dorsal orientations, while providing maximal access to target sites for microinjection. Injection of rhodamine dextran was used to demonstrate the utility of this device for precise microinjection of multiple anatomical targets.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping