PUBLICATION

Microstructured Surface Arrays for Injection of Zebrafish Larvae

Authors
Ellett, F., Irimia, D.
ID
ZDB-PUB-170204-1
Date
2017
Source
Zebrafish   14(2): 140-145 (Journal)
Registered Authors
Ellett, Felix
Keywords
infection, larvae, microinjection, microstructures, xenotransplantation
MeSH Terms
  • Animals
  • Laboratory Animal Science
  • Larva
  • Microinjections/methods
  • Microinjections/veterinary*
  • Silicones
  • Zebrafish/anatomy & histology*
PubMed
28151697 Full text @ Zebrafish
Abstract
Microinjection of zebrafish larvae is an essential technique for delivery of treatments, dyes, microbes, and xenotransplantation into various tissues. Although a number of casts are available to orient embryos at the single-cell stage, no device has been specifically designed to position hatching-stage larvae for microinjection of different tissues. In this study, we present a reusable silicone device consisting of arrayed microstructures, designed to immobilize 2 days postfertilization larvae in lateral, ventral, and dorsal orientations, while providing maximal access to target sites for microinjection. Injection of rhodamine dextran was used to demonstrate the utility of this device for precise microinjection of multiple anatomical targets.
Genes / Markers
Figures
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Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes