PUBLICATION
Analysis of myelinated axon formation in zebrafish
- Authors
- D'Rozario, M., Monk, K.R., Petersen, S.C.
- ID
- ZDB-PUB-170129-14
- Date
- 2017
- Source
- Methods in cell biology 138: 383-414 (Chapter)
- Registered Authors
- Petersen, Sarah C.
- Keywords
- Myelination, Oligodendrocyte, Remyelination, Schwann cell
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Axons/metabolism*
- Humans
- Larva/metabolism
- Molecular Biology/methods*
- Myelin Sheath/genetics
- Myelin Sheath/metabolism*
- Neuroglia/metabolism*
- Neuroglia/physiology
- Zebrafish/genetics*
- Zebrafish/growth & development
- Zebrafish/metabolism
- PubMed
- 28129853 Full text @ Meth. Cell. Biol.
Citation
D'Rozario, M., Monk, K.R., Petersen, S.C. (2017) Analysis of myelinated axon formation in zebrafish. Methods in cell biology. 138:383-414.
Abstract
Myelin is a lipid-rich sheath formed by the spiral wrapping of specialized glial cells around axon segments. Myelinating glia allow for rapid transmission of nerve impulses and metabolic support of axons, and the absence of or disruption to myelin results in debilitating motor, cognitive, and emotional deficits in humans. Because myelin is a jawed vertebrate innovation, zebrafish are one of the simplest vertebrate model systems to study the genetics and development of myelinating glia. The morphogenetic cellular movements and genetic program that drive myelination are conserved between zebrafish and mammals, and myelin develops rapidly in zebrafish larvae, within 3-5days postfertilization. Myelin ultrastructure can be visualized in the zebrafish from larval to adult stages via transmission electron microscopy, and the dynamic development of myelinating glial cells may be observed in vivo via transgenic reporter lines in zebrafish larvae. Zebrafish are amenable to genetic and pharmacological screens, and screens for myelinating glial phenotypes have revealed both genes and drugs that promote myelin development, many of which are conserved in mammalian glia. Recently, zebrafish have been employed as a model to understand the complex dynamics of myelinating glia during development and regeneration. In this chapter, we describe these key methodologies and recent insights into mechanisms that regulate myelination using the zebrafish model.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping