ZFIN ID: ZDB-PUB-170125-4
A Four-Well Dish for High-Resolution Longitudinal Imaging of the Tail and Posterior Trunk of Larval Zebrafish
Weijts, B., Tkachenko, E., Traver, D., Groisman, A.
Date: 2017
Source: Zebrafish   14(5): 489-491 (Journal)
Registered Authors: Traver, David, Weijts, Bart
Keywords: imaging, vascular development, zebrafish
MeSH Terms:
  • Animals
  • Cell Culture Techniques
  • Image Processing, Computer-Assisted*
  • Immobilization/instrumentation*
  • Larva/anatomy & histology
  • Larva/ultrastructure
  • Microscopy, Confocal/methods*
  • Zebrafish/anatomy & histology*
  • Zebrafish/growth & development*
PubMed: 28118101 Full text @ Zebrafish
We describe the design, fabrication, and applications of a four-well dish for imaging of the trunk of larval zebrafish. The dish facilitates immobilization of anesthetized zebrafish larvae, with their tails gently pushed against a microscope cover glass, enabling longitudinal imaging at 24-72 h postfertilization using high-resolution objective lenses.