ZFIN ID: ZDB-PUB-170125-11
DND protein functions as a translation repressor during zebrafish embryogenesis
Kobayashi, M., Tani-Matsuhana, S., Ohkawa, Y., Sakamoto, H., Inoue, K.
Date: 2017
Source: Biochemical and Biophysical Research Communications   484(2): 235-240 (Journal)
Registered Authors: Inoue, Kunio
Keywords: Dead-end (DND), Primordial germ cell (PGC), Translation repression, Zebrafish
MeSH Terms:
  • Animals
  • Nanog Homeobox Protein/genetics
  • Protein Biosynthesis/physiology*
  • RNA, Messenger/genetics
  • RNA-Binding Proteins/genetics
  • RNA-Binding Proteins/physiology*
  • Repressor Proteins/physiology*
  • Zebrafish/embryology*
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/physiology*
PubMed: 28115159 Full text @ Biochem. Biophys. Res. Commun.
ABSTRACT
Germline and somatic cell distinction is regulated through a combination of microRNA and germ cell-specific RNA-binding proteins in zebrafish. An RNA-binding protein, DND, has been reported to relieve the miR-430-mediated repression of some germ plasm mRNAs such as nanos3 and tdrd7 in primordial germ cells (PGCs). Here, we showed that miR-430-mediated repression is not counteracted by the overexpression of DND protein in somatic cells. Using a λN-box B tethering assay in the embryo, we found that tethering of DND to reporter mRNA results in translation repression without affecting mRNA stability. Translation repression by DND was not dependent on another germline-specific translation repressor, Nanos3, in zebrafish embryos. Moreover, our data suggested that DND represses translation of nanog and dnd mRNAs, whereas an RNA-binding protein DAZ-like (DAZL) promotes dnd mRNA translation. Thus, our study showed that DND protein functions as a translation repressor of specific mRNAs to control PGC development in zebrafish.
ADDITIONAL INFORMATION