Systemic regulation of L-carnitine in nutritional metabolism in zebrafish, Danio rerio
- Li, J.M., Li, L.Y., Qin, X., Ning, L.J., Lu, D.L., Li, D.L., Zhang, M.L., Wang, X., Du, Z.Y.
- Scientific Reports 7: 40815 (Journal)
- Registered Authors
- Du, Zhen-Yu, Li, Dong-Liang, Li, Jia-Min, Lu, Dong-Liang, Wang, Xin, Zhang, Mei-Ling
- Animal physiology, Fat metabolism
- MeSH Terms
- Carnitine O-Palmitoyltransferase/genetics
- Carnitine O-Palmitoyltransferase/metabolism
- Dietary Supplements
- Lipid Metabolism/drug effects*
- Lipid Peroxidation/drug effects
- TOR Serine-Threonine Kinases/metabolism
- 28102299 Full text @ Sci. Rep.
Li, J.M., Li, L.Y., Qin, X., Ning, L.J., Lu, D.L., Li, D.L., Zhang, M.L., Wang, X., Du, Z.Y. (2017) Systemic regulation of L-carnitine in nutritional metabolism in zebrafish, Danio rerio. Scientific Reports. 7:40815.
Excess fat accumulation has been observed widely in farmed fish; therefore, efficient lipid-lowering factors have obtained high attention in the current fish nutrition studies. Dietary L-carnitine can increase fatty acid β-oxidation in mammals, but has produced contradictory results in different fish species. To date, the mechanisms of metabolic regulation of L-carnitine in fish have not been fully determined. The present study used zebrafish to investigate the systemic regulation of nutrient metabolism by dietary L-carnitine supplementation. L-carnitine significantly decreased the lipid content in liver and muscle, accompanied by increased concentrations of total and free carnitine in tissues. Meanwhile, L-carnitine enhanced mitochondrial β-oxidation activities and the expression of carnitine palmitoyltransferase 1 mRNA significantly, whereas it depressed the mRNA expression of adipogenesis-related genes. In addition, L-carnitine caused higher glycogen deposition in the fasting state, and increased and decreased the mRNA expressions of gluconeogenesis-related and glycolysis-related genes, respectively. L-carnitine also increased the hepatic expression of mTOR in the feeding state. Taken together, dietary L-carnitine supplementation decreased lipid deposition by increasing mitochondrial fatty acid β-oxidation, and is likely to promote protein synthesis. However, the L-carnitine-enhanced lipid catabolism would cause a decrease in glucose utilization. Therefore, L-carnitine has comprehensive effects on nutrient metabolism in fish.
Errata / NotesCorrigendum.
Genes / Markers
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Engineered Foreign Genes