PUBLICATION
Hyperspectral phasor analysis enables multiplexed 5D in vivo imaging
- Authors
- Cutrale, F., Trivedi, V., Trinh, L.A., Chiu, C.L., Choi, J.M., Artiga, M.S., Fraser, S.E.
- ID
- ZDB-PUB-170110-2
- Date
- 2017
- Source
- Nature Methods 14(2): 149-152 (Journal)
- Registered Authors
- Fraser, Scott E., Trinh, Le
- Keywords
- Developmental biology, Fluorescence imaging, Image processing
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Color
- Embryo, Nonmammalian
- Fourier Analysis
- Green Fluorescent Proteins/genetics
- Green Fluorescent Proteins/metabolism
- Image Processing, Computer-Assisted
- Software*
- Time-Lapse Imaging/methods*
- Zebrafish/embryology
- Zebrafish/genetics
- PubMed
- 28068315 Full text @ Nat. Methods
Citation
Cutrale, F., Trivedi, V., Trinh, L.A., Chiu, C.L., Choi, J.M., Artiga, M.S., Fraser, S.E. (2017) Hyperspectral phasor analysis enables multiplexed 5D in vivo imaging. Nature Methods. 14(2):149-152.
Abstract
Time-lapse imaging of multiple labels is challenging for biological imaging as noise, photobleaching and phototoxicity compromise signal quality, while throughput can be limited by processing time. Here, we report software called Hyper-Spectral Phasors (HySP) for denoising and unmixing multiple spectrally overlapping fluorophores in a low signal-to-noise regime with fast analysis. We show that HySP enables unmixing of seven signals in time-lapse imaging of living zebrafish embryos.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping