PUBLICATION

Hyperspectral phasor analysis enables multiplexed 5D in vivo imaging

Authors
Cutrale, F., Trivedi, V., Trinh, L.A., Chiu, C.L., Choi, J.M., Artiga, M.S., Fraser, S.E.
ID
ZDB-PUB-170110-2
Date
2017
Source
Nature Methods   14(2): 149-152 (Journal)
Registered Authors
Fraser, Scott E., Trinh, Le
Keywords
Developmental biology, Fluorescence imaging, Image processing
MeSH Terms
  • Animals
  • Animals, Genetically Modified
  • Color
  • Embryo, Nonmammalian
  • Fourier Analysis
  • Green Fluorescent Proteins/genetics
  • Green Fluorescent Proteins/metabolism
  • Image Processing, Computer-Assisted
  • Software*
  • Time-Lapse Imaging/methods*
  • Zebrafish/embryology
  • Zebrafish/genetics
PubMed
28068315 Full text @ Nat. Methods
Abstract
Time-lapse imaging of multiple labels is challenging for biological imaging as noise, photobleaching and phototoxicity compromise signal quality, while throughput can be limited by processing time. Here, we report software called Hyper-Spectral Phasors (HySP) for denoising and unmixing multiple spectrally overlapping fluorophores in a low signal-to-noise regime with fast analysis. We show that HySP enables unmixing of seven signals in time-lapse imaging of living zebrafish embryos.
Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes