PUBLICATION
Inhibiting effects of rhynchophylline on methamphetamine-dependent zebrafish are related with the expression of tyrosine hydroxylase (TH)
- Authors
- Zhu, C., Liu, W., Luo, C., Liu, Y., Li, C., Fang, M., Lin, Y., Ou, J., Chen, M., Zhu, D., Yung, K.K., Mo, Z.
- ID
- ZDB-PUB-170109-3
- Date
- 2017
- Source
- Fitoterapia 117: 47-51 (Journal)
- Registered Authors
- Liu, Wei, Yung, Ken KL
- Keywords
- Ketamine hydrochloride (PubChem CID: 15851), Methamphetamine dependence, Methamphetamine hydrochloride (PubChem CID: 10836), Rhynchophylline, Rhynchophylline (PubChem CID: 5281408), Tyrosine hydroxylase (TH)
- MeSH Terms
-
- Behavior, Animal/drug effects
- Male
- Conditioning, Operant/drug effects*
- Methamphetamine/pharmacology*
- Mesencephalon/cytology
- Mesencephalon/enzymology
- Tyrosine 3-Monooxygenase/metabolism*
- Ketamine/pharmacology
- Amphetamine-Related Disorders
- Indole Alkaloids/pharmacology*
- Zebrafish*
- Animals
- Neurons/enzymology
- PubMed
- 28063836 Full text @ Fitoterapia
Citation
Zhu, C., Liu, W., Luo, C., Liu, Y., Li, C., Fang, M., Lin, Y., Ou, J., Chen, M., Zhu, D., Yung, K.K., Mo, Z. (2017) Inhibiting effects of rhynchophylline on methamphetamine-dependent zebrafish are related with the expression of tyrosine hydroxylase (TH). Fitoterapia. 117:47-51.
Abstract
In this study, to study the effect of rhynchophylline on TH in midbrain of methamphetamine-induced conditioned place preference (CPP) adult zebrafish, place preference adult zebrafish models were established by methamphetamine (40μg/g) and the expression of TH was observed by immunohistochemistry technique and Western blot. Ketamine (150μg/g), high dose of rhynchophylline (100μg/g) group can significantly reduce the place preference; immunohistochemistry results showed that the number of TH-positive neurons in midbrain was increased in the methamphetamine model group, whereas less TH-positive neurons were found in the ketamine group and high dosage rhynchophylline group. Western blot results showed that the expression of TH protein was significantly increased in the model group, whereas less expression was found in the ketamine group, high dosage rhynchophylline group. Our data pointed out that TH plays an important role in the formation of methamphetamine-induced place preference in adult zebrafish. Rhynchophylline reversed the expression of TH in the midbrain demonstrates the potential effect of mediates methamphetamine induced rewarding effect.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping