PUBLICATION
Responses of the Proteome and Metabolome in Livers of Zebrafish Exposed Chronically to Environmentally Relevant Concentrations of Microcystin-LR
- Authors
- Chen, L., Hu, Y., He, J., Chen, J., Giesy, J.P., Xie, P.
- ID
- ZDB-PUB-161224-17
- Date
- 2017
- Source
- Environmental science & technology 51(1): 596-607 (Journal)
- Registered Authors
- Chen, Jun
- Keywords
- none
- MeSH Terms
-
- Animals
- Liver/metabolism
- Male
- Metabolome
- Microcystins/toxicity
- Proteome/metabolism*
- Proteomics
- Zebrafish/metabolism*
- PubMed
- 28005350 Full text @ Env. Sci. Tech.
Citation
Chen, L., Hu, Y., He, J., Chen, J., Giesy, J.P., Xie, P. (2017) Responses of the Proteome and Metabolome in Livers of Zebrafish Exposed Chronically to Environmentally Relevant Concentrations of Microcystin-LR. Environmental science & technology. 51(1):596-607.
Abstract
In this study, for the first time, changes in expressions of proteins and profiles of metabolites in liver of the small, freshwater fish [Formula: see text] (zebrafish) were investigated after long-term exposure to environmentally relevant concentrations of microcystin-LR (MC-LR). Male zebrafish were exposed via water to 1 or 10 μg MC-LR/L for 90 days, and iTRAQ-based proteomics and 1H NMR-based metabolomics were employed. Histopathological observations showed that MC-LR caused damage to liver, and the effects were more pronounced in fish exposed to 10 μg MC-LR/L. Metabolomic analysis also showed alterations of hepatic function, which included changes in a number of metabolic pathways, including small molecules involved in energy, glucose, lipids, and amino acids metabolism. Concentrations of lactate were significantly greater in individuals exposed to MC-LR than in unexposed controls. This indicated a shift toward anaerobic metabolism, which was confirmed by impaired respiration in mitochondria. Proteomics revealed that MC-LR significantly influenced multiple proteins, including those involved in folding of proteins and metabolism. Endoplasmic reticulum stress contributed to disturbance of metabolism of lipids in liver of zebrafish exposed to MC-LR. Identification of proteins and metabolites in liver of zebrafish responsive to MC-LR provides insights into mechanisms of chronic toxicity of MCs.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping