PUBLICATION

Tbx20 drives cardiac progenitor formation and cardiomyocyte proliferation in zebrafish

Authors

Lu, F., Langenbacher, A., Chen, J.N.

ID

ZDB-PUB-161215-24

Date

2017

Source

Developmental Biology 421(2): 139-148 (Journal)

Registered Authors

Chen, Jau-Nian, Langenbacher, Adam

Keywords

Tbx20, cardiac progenitor, cardiogenesis, cardiomyocyte proliferation, heart development, zebrafish

MeSH Terms

Animals
Embryo, Nonmammalian/cytology
Embryo, Nonmammalian/metabolism
Zebrafish/embryology*
Zebrafish/genetics
Myocardium/cytology
Codon, Nonsense/genetics
Mutation/genetics
Gene Expression Regulation, Developmental
Protein Binding/genetics
Stem Cells/cytology*
Stem Cells/metabolism
Apoptosis/genetics
Zebrafish Proteins/chemistry
Zebrafish Proteins/genetics
Zebrafish Proteins/metabolism*
DNA/metabolism
T-Box Domain Proteins/chemistry
T-Box Domain Proteins/genetics
T-Box Domain Proteins/metabolism*
Transcriptional Activation/genetics
Cell Proliferation
Organogenesis*/genetics
Base Sequence
Myocytes, Cardiac/cytology*
Myocytes, Cardiac/metabolism
Cell Count
Protein Domains
Cloning, Molecular

(all 29)

PubMed

27940156 Full text @ Dev. Biol.

Abstract

Tbx20 is a T-box transcription factor that plays essential roles in the development and maintenance of the heart. Although it is expressed by cardiac progenitors in all species examined, an involvement of Tbx20 in cardiac progenitor formation in vertebrates has not been previously described. Here we report the identification of a zebrafish tbx20 mutation that results in an inactive, truncated protein lacking any functional domains. The cardiac progenitor population is strongly diminished in this mutant, leading to the formation of a small, stretched-out heart. We found that overexpression of Tbx20 results in an enlarged heart with significantly more cardiomyocytes. Interestingly, this increase in cell number is caused by both enhanced cardiac progenitor cell formation and the proliferation of differentiated cardiomyocytes, and is dependent upon the activity of Tbx20's T-box and transcription activation domains. Together, our findings highlight a previously unappreciated role for Tbx20 in promoting cardiac progenitor formation in vertebrates and reveal a novel function for its activation domain in cardiac cell proliferation during embryogenesis.

Genes / Markers

Figures

Show all Figures

Expression

Phenotype

Fish	Conditions	Stage	Phenotype	Figure
chb2Tg; la2368Tg	heat shock	Prim-25	cardiac muscle cell cell population proliferation increased occurrence, abnormal	Fig. 6
chb2Tg; la2368Tg	heat shock	Prim-25	cardiac muscle cell increased amount, abnormal	Fig. 6
la2368Tg	heat shock	10-13 somites	anterior lateral plate mesoderm heart primordium mef2ca expression increased amount, abnormal	Fig. 6
la2368Tg	heat shock	10-13 somites	anterior lateral plate mesoderm heart primordium nkx2.5 expression increased amount, abnormal	Fig. 6
la2368Tg	heat shock	14-19 somites	anterior lateral plate mesoderm heart primordium myl7 expression increased amount, abnormal	Fig. 6
tbx20^{la1110/la1110}	control	10-13 somites	anterior lateral plate mesoderm heart primordium mef2ca expression decreased amount, abnormal	Fig. 2
tbx20^{la1110/la1110}	control	10-13 somites	anterior lateral plate mesoderm heart primordium nkx2.5 expression decreased amount, abnormal	Fig. 3
tbx20^{la1110/la1110}	control	10-13 somites	anterior lateral plate mesoderm heart primordium nkx2.5 expression decreased amount, abnormal	Fig. 2
tbx20^{la1110/la1110}	control	10-13 somites	anterior lateral plate mesoderm heart primordium tbx20 expression decreased amount, abnormal	Fig. 4
tbx20^{la1110/la1110}	control	10-13 somites	heart primordium apoptotic process occurrence, normal	Fig. 3

1 - 10 of 31

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Mutations / Transgenics

Allele	Construct	Type	Affected Genomic Region
chb2Tg	Tg(myl7:NLS-EGFP)	Transgenic Insertion
la1110		Point Mutation	tbx20
la2368Tg	Tg(hsp70l:GAL4,UAS:tbx20,mCherry)	Transgenic Insertion
twu34Tg	Tg(myl7:EGFP)	Transgenic Insertion

1 - 4 of 4

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Human Disease / Model

Sequence Targeting Reagents

Fish

Fish
chb2Tg
chb2Tg; la2368Tg
la2368Tg
tbx20^{la1110/la1110}
tbx20^{la1110/la1110}; twu34Tg
twu34Tg
WT

Antibodies

Orthology

Engineered Foreign Genes

Marker	Marker Type	Name
EGFP	EFG	EGFP
GAL4	EFG	GAL4
mCherry	EFG	mCherry

Mapping

Lu et al., 2017 ZDB-PUB-161215-24 PMID:27940156

Tbx20 drives cardiac progenitor formation and cardiomyocyte proliferation in zebrafish

Lu et al., 2017

ZDB-PUB-161215-24
PMID:27940156