ZFIN ID: ZDB-PUB-161208-4
Analyzing ERK Signal Dynamics During Zebrafish Somitogenesis
Matsui, T., Bessho, Y.
Date: 2017
Source: Methods in molecular biology (Clifton, N.J.)   1487: 367-378 (Chapter)
Registered Authors: Bessho, Yasumasa, Matsui, Takaaki
Keywords: FGF, Immunohistochemistry, MAPK, Quantitative analysis, Somite, Zebrafish
MeSH Terms:
  • Animals
  • Embryo, Nonmammalian
  • Embryonic Development
  • Fibroblast Growth Factors/metabolism
  • Immunohistochemistry
  • In Situ Hybridization
  • MAP Kinase Signaling System*
  • Microscopy, Confocal
  • Somites/embryology*
  • Zebrafish/embryology*
  • Zebrafish/physiology*
PubMed: 27924581 Full text @ Meth. Mol. Biol.
ABSTRACT
During vertebrate development, Erk is activated and regulates multiple cellular processes such as cell growth, differentiation, migration, and adhesion in a spatiotemporal manner. Whole-mount immunohistochemistry using antibodies against diphosphorylated Erk (p-Erk; active form of Erk) is a very useful method for understanding the spatial and temporal patterns of Erk activity during embryonic development. However, the fixation step of this method stops embryo development at a certain time point, making it very difficult to observe and interpret Erk activity dynamics. In this chapter, we describe a strategy that combines immunohistochemistry and quantitative analyses of multiple fixed embryos to reconstruct Erk activity dynamics during zebrafish somitogenesis.
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