PUBLICATION
Analyzing ERK Signal Dynamics During Zebrafish Somitogenesis
- Authors
- Matsui, T., Bessho, Y.
- ID
- ZDB-PUB-161208-4
- Date
- 2017
- Source
- Methods in molecular biology (Clifton, N.J.) 1487: 367-378 (Chapter)
- Registered Authors
- Bessho, Yasumasa, Matsui, Takaaki
- Keywords
- FGF, Immunohistochemistry, MAPK, Quantitative analysis, Somite, Zebrafish
- MeSH Terms
-
- Fibroblast Growth Factors/metabolism
- Microscopy, Confocal
- Somites/embryology*
- MAP Kinase Signaling System*
- Zebrafish/embryology*
- Zebrafish/physiology*
- In Situ Hybridization
- Embryonic Development
- Embryo, Nonmammalian
- Animals
- Immunohistochemistry
- PubMed
- 27924581 Full text @ Meth. Mol. Biol.
Citation
Matsui, T., Bessho, Y. (2017) Analyzing ERK Signal Dynamics During Zebrafish Somitogenesis. Methods in molecular biology (Clifton, N.J.). 1487:367-378.
Abstract
During vertebrate development, Erk is activated and regulates multiple cellular processes such as cell growth, differentiation, migration, and adhesion in a spatiotemporal manner. Whole-mount immunohistochemistry using antibodies against diphosphorylated Erk (p-Erk; active form of Erk) is a very useful method for understanding the spatial and temporal patterns of Erk activity during embryonic development. However, the fixation step of this method stops embryo development at a certain time point, making it very difficult to observe and interpret Erk activity dynamics. In this chapter, we describe a strategy that combines immunohistochemistry and quantitative analyses of multiple fixed embryos to reconstruct Erk activity dynamics during zebrafish somitogenesis.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping