PUBLICATION

Analyzing ERK Signal Dynamics During Zebrafish Somitogenesis

Authors
Matsui, T., Bessho, Y.
ID
ZDB-PUB-161208-4
Date
2017
Source
Methods in molecular biology (Clifton, N.J.)   1487: 367-378 (Chapter)
Registered Authors
Bessho, Yasumasa, Matsui, Takaaki
Keywords
FGF, Immunohistochemistry, MAPK, Quantitative analysis, Somite, Zebrafish
MeSH Terms
  • Animals
  • Embryo, Nonmammalian
  • Embryonic Development
  • Fibroblast Growth Factors/metabolism
  • Immunohistochemistry
  • In Situ Hybridization
  • MAP Kinase Signaling System*
  • Microscopy, Confocal
  • Somites/embryology*
  • Zebrafish/embryology*
  • Zebrafish/physiology*
PubMed
27924581 Full text @ Meth. Mol. Biol.
Abstract
During vertebrate development, Erk is activated and regulates multiple cellular processes such as cell growth, differentiation, migration, and adhesion in a spatiotemporal manner. Whole-mount immunohistochemistry using antibodies against diphosphorylated Erk (p-Erk; active form of Erk) is a very useful method for understanding the spatial and temporal patterns of Erk activity during embryonic development. However, the fixation step of this method stops embryo development at a certain time point, making it very difficult to observe and interpret Erk activity dynamics. In this chapter, we describe a strategy that combines immunohistochemistry and quantitative analyses of multiple fixed embryos to reconstruct Erk activity dynamics during zebrafish somitogenesis.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping