PUBLICATION
The Zebrafish as a Model for Human Bacterial Infections
- Authors
- Neely, M.N.
- ID
- ZDB-PUB-161204-1
- Date
- 2017
- Source
- Methods in molecular biology (Clifton, N.J.) 1535: 245-266 (Chapter)
- Registered Authors
- Neely, Melody N.
- Keywords
- Animal model, Histology, Infection, Microinjection, Pathogenesis, Streptococcus infection, Zebrafish
- MeSH Terms
-
- Animals
- Bacterial Infections/microbiology*
- Bacterial Infections/pathology
- Biopsy
- Disease Models, Animal
- Humans
- Immunohistochemistry
- Larva
- Microinjections
- Microscopy, Fluorescence/methods
- Streptococcal Infections/microbiology
- Streptococcal Infections/pathology
- Zebrafish/microbiology*
- PubMed
- 27914084 Full text @ Meth. Mol. Biol.
Citation
Neely, M.N. (2017) The Zebrafish as a Model for Human Bacterial Infections. Methods in molecular biology (Clifton, N.J.). 1535:245-266.
Abstract
The development of the zebrafish (Danio rerio) infectious disease model has provided new insights and information into pathogenesis. Many of these new discoveries would not have been possible using a typical mammalian model. The advantages of using this model are many and in the last 15 years the model has been exploited for the analysis of many different pathogens. Here, we describe in detail how to perform a bacterial infection using either the adult zebrafish or zebrafish larvae using microinjection. Multiple methods of analysis are described that can be used to address specific questions pertaining to disease progression and the interactions with the immune system.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping