PUBLICATION
Internal epitope tagging informed by relative lack of sequence conservation
- Authors
- Burg, L., Zhang, K., Bonawitz, T., Grajevskaja, V., Bellipanni, G., Waring, R., Balciunas, D.
- ID
- ZDB-PUB-161129-4
- Date
- 2016
- Source
- Scientific Reports 6: 36986 (Journal)
- Registered Authors
- Balciunas, Darius, Bellipanni, Gianfranco
- Keywords
- CRISPR-Cas9 genome editing, Genetic engineering, Zebrafish
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Antibodies/genetics
- Conserved Sequence/genetics*
- Epitopes/genetics*
- Proteins/genetics
- Sequence Alignment/methods
- Zebrafish
- PubMed
- 27892520 Full text @ Sci. Rep.
Citation
Burg, L., Zhang, K., Bonawitz, T., Grajevskaja, V., Bellipanni, G., Waring, R., Balciunas, D. (2016) Internal epitope tagging informed by relative lack of sequence conservation. Scientific Reports. 6:36986.
Abstract
Many experimental techniques rely on specific recognition and stringent binding of proteins by antibodies. This can readily be achieved by introducing an epitope tag. We employed an approach that uses a relative lack of evolutionary conservation to inform epitope tag site selection, followed by integration of the tag-coding sequence into the endogenous locus in zebrafish. We demonstrate that an internal epitope tag is accessible for antibody binding, and that tagged proteins retain wild type function.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping