PUBLICATION
[Effect of rhynchophylline on behaviors of methamphetamine-dependent zebrafish and the mechanism]
- Authors
- Chen, Y.F., Peng, J., Fang, M., Liu, Y., Nie, L.H., Mo, Z.X., Zhu, L.L.
- ID
- ZDB-PUB-161125-6
- Date
- 2016
- Source
- Nan fang yi ke da xue xue bao = Journal of Southern Medical University 36: 1541-1545 (Journal)
- Registered Authors
- Chen, Y.F., Mo, Z.X.
- Keywords
- none
- MeSH Terms
-
- Animals
- Behavior, Animal/drug effects*
- Brain/drug effects*
- Indole Alkaloids/pharmacology*
- Ketamine/pharmacology
- Methamphetamine/pharmacology*
- Zebrafish/physiology*
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- PubMed
- 27881347
Citation
Chen, Y.F., Peng, J., Fang, M., Liu, Y., Nie, L.H., Mo, Z.X., Zhu, L.L. (2016) [Effect of rhynchophylline on behaviors of methamphetamine-dependent zebrafish and the mechanism]. Nan fang yi ke da xue xue bao = Journal of Southern Medical University. 36:1541-1545.
Abstract
Objective To observe the effect of rhynchophylline on methamphetamine-dependent zebrafish and explore the possible mechanism.
Methods Zebrafish were divided into control group, amphetamine group, low- (50 mg/kg) and high (100 mg/kg)-dose rhynchophylline groups, and ketamine (150 mg/kg) group. Conditioned place preference (CPP) was induced in zebrafish with methamphetamine, and the staying time in the drug box and the tracking map of the zebrafish were observed with Noldus Ethovision XT system. The protein expressions of TH, NR2B and GLUR2 in the brain of zebrafish with CPP were detected with Western blotting.
Results Compared with the control group, zebrafish in methamphetamine group showed significant variations in the staying time and swimming distance in the drug box after conditioning (P<0.05) with obvious alterations of NR2B, TH and GLUR2 expressions in the brain (P<0.05). Treatment of methamphetamine-dependent zebrafish with high-dose rhynchophylline significantly reduced the variations in the staying time and swimming distance in the drug box (P<0.05) and in the expressions of NR2B, TH and GLUR2 in the brain (P<0.05).
Conclusion Rhynchophylline can inhibit methamphetamine dependence in zebrafish, the mechanism of which may involve the expressions of TH, NR2B and GLUR2 proteins in the brain.
Errata / Notes
Article in Chinese.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping