PUBLICATION

Role of ectonucleotide pyrophosphatase/phosphodiesterase 2 in the midline axis formation of zebrafish

Authors
Frisca, F., Colquhoun, D., Goldshmit, Y., Änkö, M.L., Pébay, A., Kaslin, J.
ID
ZDB-PUB-161125-1
Date
2016
Source
Scientific Reports   6: 37678 (Journal)
Registered Authors
Goldshmit, Yona, Kaslin, Jan
Keywords
Embryology, Lipid signalling
MeSH Terms
  • Animals
  • Body Patterning*
  • Cell Movement
  • Cilia/metabolism
  • Embryo, Nonmammalian/enzymology*
  • Embryonic Development/genetics
  • Gene Expression Regulation, Developmental
  • Organogenesis
  • Phenotype
  • Phosphoric Diester Hydrolases/genetics
  • Phosphoric Diester Hydrolases/metabolism*
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Zebrafish/metabolism*
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
  • rho GTP-Binding Proteins/metabolism
  • rho-Associated Kinases/metabolism
PubMed
27883058 Full text @ Sci. Rep.
Abstract
Lysophosphatidic acid (LPA) is a unique bioactive lysophospholipid that induces pleiotropic effects in various cell types and organisms by acting on its specific receptors. LPA is mainly synthetised extracellularly by the ectonucleotide pyrophosphatase/phosphodiesterase 2/autotaxin (enpp2). Altered LPA signalling is associated with embryonic abnormalities, suggesting critical roles for LPA during development. However, the role of LPA signalling during early embryogenesis is not well established. We demonstrate that enpp2/LPA signalling in the early zebrafish embryo results in altered axis and midline formation, defects in left right (L-R) patterning, ciliogenesis of the Kupffer's vesicle (KV), through the modulation of cell migration during gastrulation in a lpar1-3 Rho/ROCK-dependant manner. Overall, this study demonstrates an essential role of enpp2/LPA signalling during early embryogenesis.
Genes / Markers
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Human Disease / Model
Sequence Targeting Reagents
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Mapping