PUBLICATION

Myosin phosphatase Fine-tunes Zebrafish Motoneuron Position during Axonogenesis

Authors
Bremer, J., Granato, M.
ID
ZDB-PUB-161118-2
Date
2016
Source
PLoS Genetics   12: e1006440 (Journal)
Registered Authors
Granato, Michael
Keywords
Axons, Embryos, Myosins, Notochords, Spinal cord, Muscle cells, Motor neurons, Zebrafish
MeSH Terms
  • Animals
  • Axons*
  • Embryonic Development/genetics*
  • Gene Expression Regulation, Developmental
  • Motor Neurons/cytology
  • Motor Neurons/metabolism
  • Muscle Cells/cytology
  • Muscle Cells/metabolism
  • Myosin-Light-Chain Phosphatase/biosynthesis
  • Myosin-Light-Chain Phosphatase/genetics*
  • Neurogenesis/genetics*
  • Notochord/growth & development
  • Spinal Cord/growth & development
  • Zebrafish/genetics
  • Zebrafish/growth & development
PubMed
27855159 Full text @ PLoS Genet.
Abstract
During embryogenesis the spinal cord shifts position along the anterior-posterior axis relative to adjacent tissues. How motor neurons whose cell bodies are located in the spinal cord while their axons reside in adjacent tissues compensate for such tissue shift is not well understood. Using live cell imaging in zebrafish, we show that as motor axons exit from the spinal cord and extend through extracellular matrix produced by adjacent notochord cells, these cells shift several cell diameters caudally. Despite this pronounced shift, individual motoneuron cell bodies stay aligned with their extending axons. We find that this alignment requires myosin phosphatase activity within motoneurons, and that mutations in the myosin phosphatase subunit mypt1 increase myosin phosphorylation causing a displacement between motoneuron cell bodies and their axons. Thus, we demonstrate that spinal motoneurons fine-tune their position during axonogenesis and we identify the myosin II regulatory network as a key regulator.
Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes