ZFIN ID: ZDB-PUB-161110-8
Chitosan nanoparticles and their Tween 80 modified counterparts disrupt the developmental profile of zebrafish embryos
Yuan, Z., Li, Y., Hu, Y., You, J., Higashisaka, K., Nagano, K., Tsutsumi, Y., Gao, J.
Date: 2016
Source: International journal of pharmaceutics   515(1-2): 644-656 (Journal)
Registered Authors: Li, Ying
Keywords: Chitosan nanoparticles, Developmental, Developmental neurotoxicity, Tween 80, Zebrafish
MeSH Terms:
  • Animals
  • Chitosan/adverse effects*
  • Chitosan/chemistry
  • Embryonic Development/drug effects*
  • Larva/drug effects
  • Larva/growth & development
  • Muscles/drug effects
  • Nanoparticles/adverse effects*
  • Nanoparticles/chemistry
  • Neurons/drug effects
  • Polysorbates/adverse effects*
  • Polysorbates/chemistry
  • Zebrafish/growth & development*
PubMed: 27826026 Full text @ Int J Pharm
ABSTRACT
Chitosan nanoparticles (CS-NPs) and their Tween 80 modified counterparts (TmCS-NPs) are among the most commonly used brain-targeted vehicles. However, their potential developmental toxicity is poorly understood. In this study, zebrafish embryos are introduced as an in vivo platform. Both NPs showed a dose-dependent increase in developmental toxicity (decreased hatching rate, increased mortality and incidences of malformation). Neurobehavioral changes included decreased spontaneous movement in TmCS-NP treated embryos and hyperactive effect in CS-NP treated larvae. Both NPs remarkably inhibited axonal development of primary and secondary motor neurons, and affected the muscle structure. Overall, this study demonstrated that CS-NPs and TmCS-NPs could affect embryonic development, disrupt neurobehavior of zebrafish larvae and affect muscle and neuron development, suggesting more attention on biodegradable chitosan nanoparticles.
ADDITIONAL INFORMATION