PUBLICATION
Possible molecular mechanism underlying cadmium-induced circadian rhythms disruption in zebrafish
- Authors
- Xiao, B., Chen, T.M., Zhong, Y.
- ID
- ZDB-PUB-161028-11
- Date
- 2016
- Source
- Biochemical and Biophysical Research Communications 481(3-4): 201-205 (Journal)
- Registered Authors
- Zhong, Yingbin
- Keywords
- Cadmium, Circadian rhythms, Microarray data, Zebrafish, mRNA level
- MeSH Terms
-
- Animals
- Cadmium/toxicity*
- Circadian Rhythm/drug effects*
- Circadian Rhythm/genetics
- Gene Expression Profiling
- Gene Expression Regulation/drug effects
- Gene Ontology
- RNA, Messenger/genetics
- RNA, Messenger/metabolism
- Reproducibility of Results
- Signal Transduction/drug effects
- Signal Transduction/genetics
- Zebrafish/genetics
- Zebrafish/physiology*
- PubMed
- 27784643 Full text @ Biochem. Biophys. Res. Commun.
Citation
Xiao, B., Chen, T.M., Zhong, Y. (2016) Possible molecular mechanism underlying cadmium-induced circadian rhythms disruption in zebrafish. Biochemical and Biophysical Research Communications. 481(3-4):201-205.
Abstract
This study was aimed to explore the mechanisms underlying cadmium-induced circadian rhythms disruption. Two groups of zebrafish larvae treated with or without 5 ppm CdCl2 were incubated in a photoperiod of 14-h light/10-h dark conditions. The mRNA levels of clock1a, bmal1b, per2 and per1b in two groups were determined. Microarray data were generated in two group of samples. Differential expression of genes were identified and the changes in expression level for some genes were validated by RT-PCR. Finally, Gene Ontology functional and KEGG pathway enrichment analysis of differentially expressed genes (DEGs) were performed. In comparison with normal group, the mRNA levels of clock1a, bmal1b, and per2 were significantly changed and varied over the circadian cycle in CdCl2-treated group. DEGs were obtained from the light (84 h, ZT12) and dark (88 h, ZT16) phase. In addition, G-protein coupled receptor protein signaling pathway and immune response were both enriched by DEGs in both groups. While, proteolysis and amino acid metabolism were found associated with DEGs in light phase, and Neuroactive ligand-receptor interaction and oxidation-reduction process were significantly enriched by DEGs in dark phase. Besides, the expression pattern of genes including hsp70l and or115-11 obtained by RT-PCR were consistent with those obtained by microarray analysis. As a consequence, cadmium could make significant effects on circadian rhythms through immune response and G protein-coupled receptor signaling pathway. Besides, between the dark and the light phase, the mechanism by which cadmium inducing disruption of circadian rhythms were different to some extent.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping