PUBLICATION
A Nuclear Role for miR-9 and Argonaute Proteins in Balancing Quiescent and Activated Neural Stem Cell States
- Authors
- Katz, S., Cussigh, D., Urbán, N., Blomfield, I., Guillemot, F., Bally-Cuif, L., Coolen, M.
- ID
- ZDB-PUB-161027-1
- Date
- 2016
- Source
- Cell Reports 17: 1383-1398 (Journal)
- Registered Authors
- Bally-Cuif, Laure, Coolen, Marion, Cussigh, Delphine, Katz, Shauna
- Keywords
- Argonaute, Notch, adult neurogenesis, miR-9, neural stem cell, quiescence, radial glia, telencephalon, zebrafish
- MeSH Terms
-
- Aging/metabolism
- Animals
- Argonaute Proteins/metabolism
- Cell Cycle*
- Cell Nucleus/metabolism
- Gene Expression Regulation
- Mice
- MicroRNAs/genetics
- MicroRNAs/metabolism*
- Models, Biological
- Neural Stem Cells/cytology*
- Neural Stem Cells/metabolism*
- Neuroglia/metabolism
- Receptors, Notch/metabolism
- Signal Transduction
- Telencephalon/metabolism
- Zebrafish/genetics*
- Zebrafish/metabolism
- PubMed
- 27783951 Full text @ Cell Rep.
Citation
Katz, S., Cussigh, D., Urbán, N., Blomfield, I., Guillemot, F., Bally-Cuif, L., Coolen, M. (2016) A Nuclear Role for miR-9 and Argonaute Proteins in Balancing Quiescent and Activated Neural Stem Cell States. Cell Reports. 17:1383-1398.
Abstract
Throughout life, adult neural stem cells (NSCs) produce new neurons and glia that contribute to crucial brain functions. Quiescence is an essential protective feature of adult NSCs; however, the establishment and maintenance of this state remain poorly understood. We demonstrate that in the adult zebrafish pallium, the brain-enriched miR-9 is expressed exclusively in a subset of quiescent NSCs, highlighting a heterogeneity within these cells, and is necessary to maintain NSC quiescence. Strikingly, miR-9, along with Argonaute proteins (Agos), is localized to the nucleus of quiescent NSCs, and manipulating their nuclear/cytoplasmic ratio impacts quiescence. Mechanistically, miR-9 permits efficient Notch signaling to promote quiescence, and we identify the RISC protein TNRC6 as a mediator of miR-9/Agos nuclear localization in vivo. We propose a conserved non-canonical role for nuclear miR-9/Agos in controlling the balance between NSC quiescence and activation, a key step in maintaining adult germinal pools.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping