PUBLICATION
Surface modification of nanostructure lipid carrier (NLC) by oleoyl-quaternized-chitosan as a mucoadhesive nanocarrier
- Authors
- Yostawonkul, J., Surassmo, S., Iempridee, T., Pimtong, W., Suktham, K., Sajomsang, W., Gonil, P., Ruktanonchai, U.R.
- ID
- ZDB-PUB-161026-2
- Date
- 2017
- Source
- Colloids and surfaces. B, Biointerfaces 149: 301-311 (Journal)
- Registered Authors
- Pimtong, Wittaya
- Keywords
- Alpha-mangostin, Mucoadhesive, Nanostructure lipid carrier, Oleoyl-quaternized-chitosan
- MeSH Terms
-
- Apoptosis/drug effects
- Caco-2 Cells
- Cell Survival/drug effects
- Chitosan/analogs & derivatives*
- Cyclin D1/genetics
- Cyclin D1/metabolism
- Cyclin-Dependent Kinase 4/genetics
- Cyclin-Dependent Kinase 4/metabolism
- Drug Carriers*
- Drug Compounding
- Drug Liberation
- Gene Expression/drug effects
- HeLa Cells
- Humans
- Hydrophobic and Hydrophilic Interactions
- Nanoparticles/chemistry*
- Nanoparticles/ultrastructure
- Particle Size
- Protein Kinase Inhibitors/chemistry
- Protein Kinase Inhibitors/pharmacology*
- Proto-Oncogene Proteins c-bcl-2/genetics
- Proto-Oncogene Proteins c-bcl-2/metabolism
- Surface Properties
- Xanthones/chemistry
- Xanthones/pharmacology*
- bcl-2-Associated X Protein/genetics
- bcl-2-Associated X Protein/metabolism
- bcl-X Protein/genetics
- bcl-X Protein/metabolism
- PubMed
- 27780087 Full text @ Colloids Surf. B Biointerfaces
Citation
Yostawonkul, J., Surassmo, S., Iempridee, T., Pimtong, W., Suktham, K., Sajomsang, W., Gonil, P., Ruktanonchai, U.R. (2017) Surface modification of nanostructure lipid carrier (NLC) by oleoyl-quaternized-chitosan as a mucoadhesive nanocarrier. Colloids and surfaces. B, Biointerfaces. 149:301-311.
Abstract
A nanostructure lipid carrier (NLC) composed of solid, and liquid lipid as a core has been developed as a delivery system for hydrophobic drug molecules. The aim of this research was to fabricate an oleoyl-quaternized-chitosan (CS)-coated NLC, where the mucoadhesive property of nanoparticles is enhanced for more efficient drug delivery. NLC loaded with alpha-mangostin (AP), a model hydrophobic drug, were fabricated using a high pressure homogenization process and subsequently coated with CS. The fabricated nanoparticles showed particle sizes in the range of 200-400nm, with low polydispersity, high physical stability and excellent encapsulation efficiency (EE>90%). Additionally, in vitro viability, cytotoxicity and ability of NLC and CS-NLC to affect apoptosis in carcinoma Caco-2 cells were determined using the Triplex assay. Gene expressiom analysis were performed using quantitative reverse transcription Polymerase Chain Reaction (RT-qPCR). Moreover, in vivo toxicological testing of NLCs was conducted in zebrafish embryos. Results indicated that CS-NLC provieded high cytotoxicity than NLC itself. In the case of AP loaded nanoparticles, NLC loaded with AP (AP-NLC), and CS-NLC loaded with AP (CS-AP-NLC) exhibited higher cytotoxicity to Caco-2 over Hela cells. These results indicate that CS-NLC shows enhanced cellular uptake but increased cytotoxicity characteristics over NLC and therefore careful optimization of dosage and loading levels in CS-NLC is needed to allow cancer cell targeting, and for exploiting the potential of these systems in cancer therapy.
Genes / Markers
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Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
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