PUBLICATION
Identification of Germ-Line Stem Cells in Zebrafish
- Authors
- Draper, B.W.
- ID
- ZDB-PUB-161014-4
- Date
- 2017
- Source
- Methods in molecular biology (Clifton, N.J.) 1463: 103-113 (Chapter)
- Registered Authors
- Draper, Bruce
- Keywords
- Confocal immunofluorescence, Fluorescent RNA in situ hybridization, Oogonial stem cell, Spermatogonial stem cell, Vasa, Zebrafish germ-line stem cells, Zebrafish ovary, Zebrafish testis, nanos2
- MeSH Terms
-
- Adult Germline Stem Cells/chemistry*
- Adult Germline Stem Cells/metabolism
- Animals
- DEAD-box RNA Helicases/metabolism
- Female
- Fluorescent Antibody Technique
- In Situ Hybridization, Fluorescence
- Male
- Oogonial Stem Cells/chemistry*
- Oogonial Stem Cells/metabolism
- Ovary/cytology
- Ovary/metabolism
- RNA-Binding Proteins/genetics*
- Testis/cytology
- Testis/metabolism
- Tissue Fixation
- Zebrafish/genetics*
- Zebrafish/metabolism
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- PubMed
- 27734351 Full text @ Meth. Mol. Biol.
Citation
Draper, B.W. (2017) Identification of Germ-Line Stem Cells in Zebrafish. Methods in molecular biology (Clifton, N.J.). 1463:103-113.
Abstract
Both male and female zebrafish have a population of germ-line stem cells that produce gametes throughout the life of the fish. These cells localize to specific regions in the gonads and can be identified because they uniquely express the nanos2 gene, which encodes a conserved regulator of translation. A method is presented here for identifying germ-line stem cells in the ovary and testis using a combined protocol for whole-mount fluorescent RNA in situ hybridization to detect nanos2 mRNA and immunofluorescence to detect the pan-germ cell marker Vasa.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping