PUBLICATION
Quercetin protects against hair cell loss in the zebrafish lateral line and guinea pig cochlea
- Authors
- Hirose, Y., Sugahara, K., Kanagawa, E., Takemoto, Y., Hashimoto, M., Yamashita, H.
- ID
- ZDB-PUB-161009-2
- Date
- 2016
- Source
- Hearing Research 342: 80-85 (Journal)
- Registered Authors
- Yamashita, Hiroshi
- Keywords
- cochlear protection, noise trauma, oxidation, screening, zebrafish
- MeSH Terms
-
- Animals
- Antioxidants/metabolism
- Auditory Threshold/drug effects
- Dose-Response Relationship, Drug
- Drug Evaluation, Preclinical
- Evoked Potentials, Auditory, Brain Stem/drug effects
- Guinea Pigs
- Hair Cells, Auditory, Outer/drug effects*
- Hair Cells, Auditory, Outer/pathology
- Hair Cells, Auditory, Outer/physiology
- Lateral Line System/drug effects*
- Lateral Line System/pathology
- Lateral Line System/physiopathology
- Male
- Neomycin/administration & dosage
- Neomycin/toxicity
- Noise/adverse effects
- Protective Agents/administration & dosage
- Protective Agents/pharmacology
- Quercetin/administration & dosage
- Quercetin/pharmacology*
- Zebrafish
- PubMed
- 27717895 Full text @ Hear. Res.
Citation
Hirose, Y., Sugahara, K., Kanagawa, E., Takemoto, Y., Hashimoto, M., Yamashita, H. (2016) Quercetin protects against hair cell loss in the zebrafish lateral line and guinea pig cochlea. Hearing Research. 342:80-85.
Abstract
Eighteen supplement drugs were screened using hair cells to determine a protective effect against the adverse effects of neomycin by using the zebrafish lateral line. The zebrafish were administered the supplement drugs one hour before neomycin exposure. One hour later, animals were fixed in paraformaldehyde. Dose-response curves were generated to evaluate the protective effect on hair cells. The screen identified 3 supplements (quercetin, catechin and tannic acid). Three minutes after exposure to neomycin, increased antioxidant activity was found in the lateral line hair cells, as determined by the analysis of oxidative stress. Quercetin decreases antioxidant activity. The identified drugs were also investigated to determine whether they protect the cochlea against noise-induced hearing loss in guinea pigs. The drugs were administered via the intraperitoneal route in the guinea pigs 3 days before and 4 days after noise exposure. Seven days after noise exposure (130-dB sound pressure level for 3 hours), the auditory brainstem response threshold shifts were assessed. We observed that the auditory brainstem response threshold shift was significantly less in the quercetin group than in the vehicle control group. The results of our study indicate that screening drugs using zebrafish can determine additional protective drugs for the inner ear.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping