PUBLICATION

Cre inducible site-specific recombination in zebrafish oligodendrocytes

Authors
Pinzon-Olejua, A., Welte, C., Chekuru, A., Bosak, V., Brand, M., Hans, S., Stuermer, C.A.
ID
ZDB-PUB-160927-4
Date
2017
Source
Developmental Dynamics : an official publication of the American Association of Anatomists   246(1): 41-49 (Journal)
Registered Authors
Brand, Michael, Chekuru, Avinash, Hans, Stefan, Stuermer, Claudia
Keywords
Cre-lox system, Oligodendrocytes, glia cell specific recombination, myelin basic protein-promoter, myelination, transgenic zebrafish
MeSH Terms
  • Zebrafish/embryology*
  • Zebrafish/metabolism
  • Oligodendroglia/metabolism*
  • Oligodendroglia/ultrastructure
  • Animals, Genetically Modified
  • Integrases/metabolism*
  • Recombination, Genetic
  • Transgenes
  • Animals
  • Myelin Sheath/metabolism*
  • Genes, Reporter
  • Myelin Basic Protein/genetics
  • Gene Expression Regulation, Developmental
  • Demyelinating Diseases
  • Promoter Regions, Genetic
(all 15)
PubMed
27666728 Full text @ Dev. Dyn.
Abstract
Background The conditional Cre/lox system has recently emerged as a valuable tool for studies on both embryonic and adult zebrafish. Temporal control and site-specific recombination is achieved by using the ligand-inducible CreER(T2) and administration of the drug tamoxifen (TAM) or its active metabolite, 4-Hydroxytamoxifen (4-OHT).
Results Here we report the generation of a transgenic zebrafish line, which expresses a mCherry-tagged variant of CreER(T2) under the control of the myelin basic protein a (mbpa) promoter. Our analysis shows that larval and adult expression of the transgene recapitulates the endogenous mbpa expression pattern in oligodendrocytes. Furthermore, combination with a Cre-dependent EGFP reporter results in EGFP-expressing oligodendrocytes in the spinal cord, brain and optic nerve in TAM or 4-OHT treated larvae and 4 months old fish, but not in untreated controls.
Conclusions The transgenic zebrafish line Tg(mbpa:mCherry-T2A-CreER(T2) ) elicits CreER(T2) expression specifically in myelinating glia cells. Cre-inducible targeted recombination of genes in oligodendrocytes will be useful to elucidate cellular and molecular mechanisms of myelination in vivo during development (myelination) and regeneration (remyelination) after injury to the central nervous system (CNS). It will also allow targeted expression and overexpression of genes of interest (transgenes) in oligodendrocytes at defined developmental and adult stages.
Genes / Markers
Figures
Figure Gallery (4 images)
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Expression
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
ck1TgTransgenic Insertion
    kn4TgTransgenic Insertion
      kn5TgTransgenic Insertion
        tud107TgTransgenic Insertion
          1 - 4 of 4
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          Human Disease / Model
          No data available
          Sequence Targeting Reagents
          No data available
          Fish
          No data available
          Antibodies
          Name Type Antigen Genes Isotypes Host Organism
          Ab1-mbppolyclonalRabbit
          1 - 1 of 1
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          Orthology
          No data available
          Engineered Foreign Genes
          Marker Marker Type Name
          CreEFGCre
          DsRed2EFGDsRed2
          EGFPEFGEGFP
          mCherryEFGmCherry
          1 - 4 of 4
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          Mapping
          No data available
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          Citation
          Pinzon-Olejua, A., Welte, C., Chekuru, A., Bosak, V., Brand, M., Hans, S., Stuermer, C.A. (2017) Cre inducible site-specific recombination in zebrafish oligodendrocytes. Developmental Dynamics : an official publication of the American Association of Anatomists. 246(1):41-49.