PUBLICATION
Characteristics of Vibrio parahaemolyticus isolates obtained from crayfish (Procambarus clarkii) in freshwater
- Authors
- Dong, X., Li, Z., Wang, X., Zhou, M., Lin, L., Zhou, Y., Li, J.
- ID
- ZDB-PUB-160914-4
- Date
- 2016
- Source
- International journal of food microbiology 238: 132-138 (Journal)
- Registered Authors
- Li, Zhi
- Keywords
- Multilocus sequence typing, Procambarus clarkii, Vibrio parahaemolyticus
- MeSH Terms
-
- Animals
- Astacoidea/microbiology*
- Evolution, Molecular
- Food Microbiology
- Fresh Water
- Gastroenteritis/microbiology
- Gastroenteritis/prevention & control
- Genetic Variation
- Genotype
- Mice
- Multilocus Sequence Typing
- Shellfish/microbiology*
- Vibrio Infections/microbiology
- Vibrio parahaemolyticus/genetics*
- Vibrio parahaemolyticus/pathogenicity
- Virulence/genetics*
- Virulence Factors/genetics
- Zebrafish
- PubMed
- 27620824 Full text @ Int. J. Food Microbiol.
Citation
Dong, X., Li, Z., Wang, X., Zhou, M., Lin, L., Zhou, Y., Li, J. (2016) Characteristics of Vibrio parahaemolyticus isolates obtained from crayfish (Procambarus clarkii) in freshwater. International journal of food microbiology. 238:132-138.
Abstract
Vibrio parahaemolyticus usually occurs in coastal areas and is generally recognized as a marine bacterium. It has become the leading cause of gastroenteritis worldwide. In the present study, 96 V. parahaemolyticus isolates were obtained from freshwater crayfish (Procambarus clarkii) and classified by multilocus sequence typing. Fifty-three sequence types (STs) were identified among the 96 isolates analyzed, 38 of which were novel STs. These isolates fell into six groups and 42 singletons, suggesting a high level of genetic diversity. Screening for 9 virulence and virulence-related genes in the isolates revealed that 40 isolates contained more than two genes with possible roles in pathogenicity. The virulence of the representative isolates VP66 (trh+, ureC+, T3SS1+, T3SS2β+, T6SS2+) and VP80 (T3SS1+, T6SS1+, T6SS2+) were further assessed in zebrafish and mouse infection model in vivo, and the tested isolates were shown to be lethal to both zebrafish and mice. These results suggest that crayfish may serve as a carrier of V. parahaemolyticus in freshwater, and that some isolates may have the potential to cause foodborne disease in humans.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping