PUBLICATION

Stereotyped initiation of retinal waves by bipolar cells via presynaptic NMDA autoreceptors

Authors
Zhang, R.W., Li, X.Q., Kawakami, K., Du, J.L.
ID
ZDB-PUB-160903-8
Date
2016
Source
Nature communications   7: 12650 (Journal)
Registered Authors
Du, Jiu Lin, Kawakami, Koichi
Keywords
Neuronal development, Retina
MeSH Terms
  • Animals
  • Electrical Synapses/physiology
  • Glutamic Acid/metabolism
  • Larva/growth & development
  • Presynaptic Terminals/metabolism
  • Receptors, N-Methyl-D-Aspartate/metabolism*
  • Retinal Bipolar Cells/metabolism*
  • Retinal Ganglion Cells/metabolism*
  • Synaptic Transmission/physiology*
  • Visual Pathways/physiology*
  • Zebrafish/embryology*
  • Zebrafish/genetics
PubMed
27586999 Full text @ Nat. Commun.
Abstract
Glutamatergic retinal waves, the spontaneous patterned neural activities propagating among developing retinal ganglion cells (RGCs), instruct the activity-dependent refinement of visuotopic maps. However, its initiation and underlying mechanism remain largely elusive. Here using larval zebrafish and multiple in vivo approaches, we discover that bipolar cells (BCs) are responsible for the generation of glutamatergic retinal waves. The wave originates from BC axon terminals (ATs) and propagates laterally to nearby BCs and vertically to downstream RGCs and the optic tectum. Its initiation is triggered by the activation of and consequent glutamate release from BC ATs, and is mediated by the N-methyl-D-aspartate subtype of glutamate receptors (NMDARs) expressed at these ATs. Intercellular asymmetry of NMDAR expression at BC ATs enables the preferential initiation of waves at the temporal retina, where BC ATs express more NMDARs. Thus, our findings indicate that glutamatergic retinal waves are initiated by BCs through a presynaptic NMDA autoreceptor-dependent process.
Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes