PUBLICATION
Cone signals in monostratified and bistratified amacrine cells of adult zebrafish retina
- Authors
- Torvund, M.M., Ma, T.S., Connaughton, V.P., Ono, F., Nelson, R.F.
- ID
- ZDB-PUB-160830-5
- Date
- 2017
- Source
- The Journal of comparative neurology 525(7): 1532-1557 (Journal)
- Registered Authors
- Connaughton, Victoria P., Ono, Fumihito
- Keywords
- 3D-reconstruction, GE4a transgenic, OFF response, ON response, ON-OFF response, RRID:SCR_001775, RRID:SCR_002368, RRID:SCR_002798, RRID:SCR_003070, RRID:SCR_008406, RRID:SCR_008593, RRID:SCR_008597, RRID:SCR_008960, RRID:SCR_011323, RRID:SCR_013318, RRID:SCR_013672, enhancer trap, inner plexiform layer, spectral model, wavelength
- MeSH Terms
-
- Amacrine Cells/cytology*
- Amacrine Cells/physiology*
- Animals
- Animals, Genetically Modified
- Imaging, Three-Dimensional
- Patch-Clamp Techniques
- Retinal Cone Photoreceptor Cells/cytology*
- Retinal Cone Photoreceptor Cells/physiology*
- Zebrafish
- PubMed
- 27570913 Full text @ J. Comp. Neurol.
Citation
Torvund, M.M., Ma, T.S., Connaughton, V.P., Ono, F., Nelson, R.F. (2017) Cone signals in monostratified and bistratified amacrine cells of adult zebrafish retina. The Journal of comparative neurology. 525(7):1532-1557.
Abstract
Strata within the inner plexiform layer (IPL) of vertebrate retinas are suspected to be distinct signaling regions. Functions performed within adult zebrafish IPL strata were examined through microelectrode recording and staining of stratified amacrine types. The stimulus protocol and analysis discriminated the pattern of input from red, green, blue, and UV cones as well as the light-response waveforms in this tetrachromatic species. A total of 36 cells were analyzed. Transient depolarizing waveforms at ON and OFF originated with bistratified amacrine types, whose dendritic planes branched either in IPL sublaminas a & b, or only within sublamina a. Monophasic-sustained depolarizing waveforms originated with types monostratified in IPL s4 (sublamina b). OFF responses hyperpolarized at onset, depolarized at offset, and in some cases depolarized during mid stimulus. These signals originated with types monostratified in s1 or s2 (sublamina a). Bistratified amacrines received depolarizing signals only from red cones, both at ON and OFF, while s4 stratified ON cells combined red and green cone signals. The s1/s2 stratified OFF cells utilized hyperpolarizing signals from red, red and green, or red and blue cones at ON, but only depolarizing red cone signals at OFF. ON and OFF depolarizing transients from red cones appear widely distributed within IPL strata. 'C-type' physiologies, depolarized by some wavelengths, hyperpolarized by others, in biphasic or triphasic spectral patterns, originated with amacrine cells monostratified in s5. Collectively cells in this stratum processed signals from all cone types. This article is protected by copyright. All rights reserved.
Errata / Notes
Correction.
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Human Disease / Model
Sequence Targeting Reagents
Fish
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