PUBLICATION
Validation of Protein Knockout in Mutant Zebrafish Lines Using In Vitro Translation Assays
- Authors
- Carter, B.S., Cortés-Campos, C., Chen, X., McCammon, J.M., Sive, H.L.
- ID
- ZDB-PUB-160823-1
- Date
- 2017
- Source
- Zebrafish 14(1): 73-76 (Journal)
- Registered Authors
- McCammon, Jasmine, Sive, Hazel
- Keywords
- none
- MeSH Terms
-
- Animals
- Animals, Genetically Modified/genetics
- Animals, Genetically Modified/metabolism*
- Cell-Free System*
- Gene Editing
- Gene Knockout Techniques/methods*
- Mutation
- Protein Biosynthesis*
- Zebrafish/genetics
- Zebrafish/metabolism*
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- PubMed
- 27548568 Full text @ Zebrafish
Citation
Carter, B.S., Cortés-Campos, C., Chen, X., McCammon, J.M., Sive, H.L. (2017) Validation of Protein Knockout in Mutant Zebrafish Lines Using In Vitro Translation Assays. Zebrafish. 14(1):73-76.
Abstract
Advances in genome-editing technology have made creation of zebrafish mutant lines accessible to the community. Experimental validation of protein knockout is a critical step in verifying null mutants, but this can be a difficult task. Absence of protein can be confirmed by Western blotting; however, this approach requires target-specific antibodies that are generally not available for zebrafish proteins. We address this issue using in vitro translation assays, a fast and standard procedure that can be easily implemented.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping