ZFIN ID: ZDB-PUB-160823-1
Validation of Protein Knockout in Mutant Zebrafish Lines Using In Vitro Translation Assays
Carter, B.S., Cortés-Campos, C., Chen, X., McCammon, J.M., Sive, H.L.
Date: 2017
Source: Zebrafish   14(1): 73-76 (Journal)
Registered Authors: McCammon, Jasmine, Sive, Hazel
Keywords: none
MeSH Terms:
  • Animals
  • Animals, Genetically Modified/genetics
  • Animals, Genetically Modified/metabolism*
  • Cell-Free System*
  • Gene Editing
  • Gene Knockout Techniques/methods*
  • Mutation
  • Protein Biosynthesis*
  • Zebrafish/genetics
  • Zebrafish/metabolism*
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
PubMed: 27548568 Full text @ Zebrafish
Advances in genome-editing technology have made creation of zebrafish mutant lines accessible to the community. Experimental validation of protein knockout is a critical step in verifying null mutants, but this can be a difficult task. Absence of protein can be confirmed by Western blotting; however, this approach requires target-specific antibodies that are generally not available for zebrafish proteins. We address this issue using in vitro translation assays, a fast and standard procedure that can be easily implemented.