PUBLICATION

Intravital assessment of myelin molecular order with polarimetric multiphoton microscopy

Authors
Turcotte, R., Rutledge, D.J., Bélanger, E., Dill, D., Macklin, W.B., Côté, D.C.
ID
ZDB-PUB-160820-11
Date
2016
Source
Scientific Reports   6: 31685 (Journal)
Registered Authors
Macklin, Wendy B.
Keywords
Biophysics, Medical research, Microscopy, Myelin biology and repair, Optical physics
MeSH Terms
  • Animals
  • Intravital Microscopy/methods*
  • Microscopy, Fluorescence, Multiphoton/methods*
  • Myelin Sheath/metabolism*
  • Zebrafish/embryology*
PubMed
27538357 Full text @ Sci. Rep.
Abstract
Myelin plays an essential role in the nervous system and its disruption in diseases such as multiple sclerosis may lead to neuronal death, thus causing irreversible functional impairments. Understanding myelin biology is therefore of fundamental and clinical importance, but no tools currently exist to describe the fine spatial organization of myelin sheaths in vivo. Here we demonstrate intravital quantification of the myelin molecular structure using a microscopy method based on polarization-resolved coherent Raman scattering. Developmental myelination was imaged noninvasively in live zebrafish. Longitudinal imaging of individual axons revealed changes in myelin organization beyond the diffraction limit. Applied to promyelination drug screening, the method uniquely enabled the identification of focal myelin regions with differential architectures. These observations indicate that the study of myelin biology and the identification of therapeutic compounds will largely benefit from a method to quantify the myelin molecular organization in vivo.
Genes / Markers
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Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping