PUBLICATION
Fiber Optic-Based Photostimulation of Larval Zebrafish
- Authors
- Arrenberg, A.B.
- ID
- ZDB-PUB-160729-6
- Date
- 2016
- Source
- Methods in molecular biology (Clifton, N.J.) 1451: 343-54 (Chapter)
- Registered Authors
- Arrenberg, Aristides
- Keywords
- Agarose, Laser, Mounting, Optic fiber, Optogenetics, Photostimulation, Zebrafish
- MeSH Terms
-
- Animals
- Fiber Optic Technology/methods*
- Larva/metabolism*
- Optogenetics/methods*
- Zebrafish/metabolism*
- PubMed
- 27464820 Full text @ Meth. Mol. Biol.
Citation
Arrenberg, A.B. (2016) Fiber Optic-Based Photostimulation of Larval Zebrafish. Methods in molecular biology (Clifton, N.J.). 1451:343-54.
Abstract
The perturbation of neural activity is a powerful experimental approach for understanding brain function. Light-gated ion channels and pumps (optogenetics) can be used to control neural activity with high temporal and spatial precision in animal models. This optogenetic approach requires suitable methods for delivering light to the brain. In zebrafish, fiber optic stimulation of agarose-embedded larvae has successfully been used in several studies to control neural activity and behavior. This approach is easy to implement and cost-efficient. Here, a protocol for fiber optic-based photostimulation of larval zebrafish is provided.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping