PUBLICATION
Zebrafish as a Model for the Study of Solid Malignancies
- Authors
- Kendall, G.C., Amatruda, J.F.
- ID
- ZDB-PUB-160729-21
- Date
- 2016
- Source
- Methods in molecular biology (Clifton, N.J.) 1451: 121-42 (Chapter)
- Registered Authors
- Amatruda, James F., Kendall, Genevieve
- Keywords
- Functional genomics, Gateway cloning, Histopathology, Solid malignancies, Tol2, Transgenesis, Zebrafish tumor model
- MeSH Terms
-
- Animals
- Animals, Genetically Modified/genetics
- Animals, Genetically Modified/metabolism*
- Carcinogenesis/genetics
- Carcinogenesis/metabolism
- Cell Transformation, Neoplastic/genetics
- Embryo, Nonmammalian/metabolism
- Gene Transfer Techniques
- Genomics/methods*
- Neoplasms/genetics
- Neoplasms/metabolism*
- Zebrafish/genetics
- Zebrafish/metabolism*
- PubMed
- 27464805 Full text @ Meth. Mol. Biol.
Citation
Kendall, G.C., Amatruda, J.F. (2016) Zebrafish as a Model for the Study of Solid Malignancies. Methods in molecular biology (Clifton, N.J.). 1451:121-42.
Abstract
Zebrafish cancer models have provided critical insight into understanding the link between aberrant developmental pathways and tumorigenesis. The unique strengths of zebrafish as compared to other vertebrate model systems include the combination of fecundity, readily available and efficient transgenesis techniques, transparency that facilitates in vivo cell lineage tracing, and amenability for high-throughput applications. In addition to early embryo readouts, zebrafish can develop tumors at ages ranging from 2 weeks old to adulthood. Tumorigenesis is driven by genetically introducing oncogenes using selected promoter/tissue-specific expression, with either mosaic expression or with the generation of a stable transgenic line. Here, we detail a research pipeline to facilitate the study of human oncogenes in zebrafish systems. The goals of this approach are to identify conserved developmental pathways that may be critical for tumor development and to create platforms for testing novel therapies.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping