PUBLICATION
Methodologies for Inducing Cardiac Injury and Assaying Regeneration in Adult Zebrafish
- Authors
- Wang, J., Poss, K.D.
- ID
- ZDB-PUB-160729-15
- Date
- 2016
- Source
- Methods in molecular biology (Clifton, N.J.) 1451: 225-35 (Chapter)
- Registered Authors
- Poss, Kenneth D., Wang, Jinhu
- Keywords
- Ablation, Cardiomyocyte, Heart, Regeneration, Surgery, Zebrafish
- MeSH Terms
-
- Heart Injuries/metabolism
- Heart Injuries/pathology
- Heart Injuries/physiopathology*
- Wound Healing/physiology
- Zebrafish
- Heart Ventricles/metabolism
- Heart Ventricles/pathology
- Heart Ventricles/physiopathology
- Myocardium/metabolism
- Myocardium/pathology
- Signal Transduction/physiology
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- Animals
- Regeneration/genetics
- Regeneration/physiology*
- Biological Assay/methods*
- Myocytes, Cardiac/cytology
- Myocytes, Cardiac/physiology
- Heart/physiology*
- Heart/physiopathology*
- PubMed
- 27464811 Full text @ Meth. Mol. Biol.
Citation
Wang, J., Poss, K.D. (2016) Methodologies for Inducing Cardiac Injury and Assaying Regeneration in Adult Zebrafish. Methods in molecular biology (Clifton, N.J.). 1451:225-35.
Abstract
The zebrafish has emerged as an important model organism for understanding the cellular and molecular mechanisms of tissue regeneration. Adult zebrafish efficiently replace cardiac muscle after partial resection of their ventricle, or after transgenic ablation of cardiomyocytes. Here, we describe methodology for inducing these injuries and assaying indicators of regeneration.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping