PUBLICATION
Studying Axonal Regeneration by Laser Microsurgery and High-Resolution Videomicroscopy
- Authors
- Xiao, Y., López-Schier, H.
- ID
- ZDB-PUB-160729-12
- Date
- 2016
- Source
- Methods in molecular biology (Clifton, N.J.) 1451: 271-80 (Chapter)
- Registered Authors
- Lopez-Schier, Hernan, Xiao, Yan
- Keywords
- Fluorescent protein, Live imaging, Microscopy, Zebrafish
- MeSH Terms
-
- Animals
- Axons/physiology*
- Brain/cytology
- Microscopy, Video/methods*
- Microsurgery/methods*
- Nerve Regeneration/physiology*
- Neurons/cytology
- Peripheral Nervous System/cytology
- Zebrafish
- PubMed
- 27464814 Full text @ Meth. Mol. Biol.
Citation
Xiao, Y., López-Schier, H. (2016) Studying Axonal Regeneration by Laser Microsurgery and High-Resolution Videomicroscopy. Methods in molecular biology (Clifton, N.J.). 1451:271-80.
Abstract
Heterogeneous and unpredictable environmental insult, disease, or trauma can affect the integrity and function of neuronal circuits, leading to irreversible neural dysfunction. The peripheral nervous system can robustly regenerate axons after damage to recover the capacity to transmit sensory information to the brain. The mechanisms that allow axonal repair remain incompletely understood. Here we present a preparation in zebrafish that combines laser microsurgery of sensory axons and videomicroscopy of neurons in multicolor transgenic specimens. This simple protocol allows controlled damage of axons and dynamic high-resolution visualization and quantification of repair.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping