ZFIN ID: ZDB-PUB-160729-12
Studying Axonal Regeneration by Laser Microsurgery and High-Resolution Videomicroscopy
Xiao, Y., López-Schier, H.
Date: 2016
Source: Methods in molecular biology (Clifton, N.J.)   1451: 271-80 (Chapter)
Registered Authors: Lopez-Schier, Hernan, Xiao, Yan
Keywords: Fluorescent protein, Live imaging, Microscopy, Zebrafish
MeSH Terms:
  • Animals
  • Axons/physiology*
  • Brain/cytology
  • Microscopy, Video/methods*
  • Microsurgery/methods*
  • Nerve Regeneration/physiology*
  • Neurons/cytology
  • Peripheral Nervous System/cytology
  • Zebrafish
PubMed: 27464814 Full text @ Meth. Mol. Biol.
Heterogeneous and unpredictable environmental insult, disease, or trauma can affect the integrity and function of neuronal circuits, leading to irreversible neural dysfunction. The peripheral nervous system can robustly regenerate axons after damage to recover the capacity to transmit sensory information to the brain. The mechanisms that allow axonal repair remain incompletely understood. Here we present a preparation in zebrafish that combines laser microsurgery of sensory axons and videomicroscopy of neurons in multicolor transgenic specimens. This simple protocol allows controlled damage of axons and dynamic high-resolution visualization and quantification of repair.