PUBLICATION
A detailed proteomic profiling of plasma membrane from zebrafish brain
- Authors
- Smidak, R., Aradska, J., Kirchberger, S., Distel, M., Sialana, F.J., Wackerlig, J., Mechtcheriakova, D., Lubec, G.
- ID
- ZDB-PUB-160728-7
- Date
- 2016
- Source
- Proteomics. Clinical applications 10(12): 1264-1268 (Journal)
- Registered Authors
- Distel, Martin, Kirchberger, Stefanie
- Keywords
- Brain, Membrane proteome, Zebrafish
- MeSH Terms
-
- Animals
- Brain/cytology*
- Brain/metabolism
- Cell Membrane/metabolism*
- Gene Ontology
- Proteomics*
- Zebrafish/metabolism*
- PubMed
- 27459904 Full text @ Proteomics Clin Appl
Citation
Smidak, R., Aradska, J., Kirchberger, S., Distel, M., Sialana, F.J., Wackerlig, J., Mechtcheriakova, D., Lubec, G. (2016) A detailed proteomic profiling of plasma membrane from zebrafish brain. Proteomics. Clinical applications. 10(12):1264-1268.
Abstract
Zebrafish (Danio rerio) is a well-established model organism in developmental biology and disease modeling. In recent years, an increasing amount of studies used zebrafish to analyze the genetic changes underlying various neurological disorders. The brain plasma membrane proteome represents the major subsets of signaling proteins and promising drug targets, but is often understudied due to traditional experimental difficulties including problems with solubility, detergent removal or low abundance. Here we report a comprehensive dataset of the proteins identified in the enriched plasma membrane of the zebrafish brain by applying sequential trypsin/chymotrypsin digestion with multidimensional LC-MS/MS. A total number of 97017 peptide groups corresponding to 9201 proteins were identified. These were annotated in various molecular functions or neurological disorders. The dataset of the current study provides a useful data source for further utilizing zebrafish in basic and clinical neuroscience.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping