PUBLICATION
Liposome-Mediated Herpes Simplex Virus Uptake Is Glycoprotein-D Receptor-Independent but Requires Heparan Sulfate
- Authors
- Burnham, L.A., Jaishankar, D., Thompson, J.M., Jones, K.S., Shukla, D., Tiwari, V.
- ID
- ZDB-PUB-160725-5
- Date
- 2016
- Source
- Frontiers in microbiology 7: 973 (Journal)
- Registered Authors
- Tiwari, Vaibhav
- Keywords
- heparan sulfate, viral entry, virus-cell interactions
- MeSH Terms
- none
- PubMed
- 27446014 Full text @ Front Microbiol
Citation
Burnham, L.A., Jaishankar, D., Thompson, J.M., Jones, K.S., Shukla, D., Tiwari, V. (2016) Liposome-Mediated Herpes Simplex Virus Uptake Is Glycoprotein-D Receptor-Independent but Requires Heparan Sulfate. Frontiers in microbiology. 7:973.
Abstract
Cationic liposomes are widely used to facilitate introduction of genetic material into target cells during transfection. This study describes a non-receptor mediated herpes simplex virus type-1 (HSV-1) entry into the Chinese hamster ovary (CHO-K1) cells that naturally lack glycoprotein D (gD)-receptors using a commercially available cationic liposome: lipofectamine. Presence of cell surface heparan sulfate (HS) increased the levels of viral entry indicating a potential role of HS in this mode of entry. Loss of viral entry in the presence of actin de-polymerizing or lysosomotropic agents suggests that this mode of entry results in the endocytosis of the lipofectamine-virus mixture. Enhancement of HSV-1 entry by liposomes was also demonstrated in vivo using a zebrafish embryo model that showed stronger infection in the eyes and other tissues. Our study provides novel insights into gD receptor independent viral entry pathways and can guide new strategies to enhance the delivery of viral gene therapy vectors or oncolytic viruses.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping