PUBLICATION
Rational Design of a GFP-Based Fluorogenic Caspase Reporter for Imaging Apoptosis In Vivo
- Authors
- To, T.L., Schepis, A., Ruiz-González, R., Zhang, Q., Yu, D., Dong, Z., Coughlin, S.R., Shu, X.
- ID
- ZDB-PUB-160725-3
- Date
- 2016
- Source
- Cell chemical biology 23: 875-82 (Journal)
- Registered Authors
- Dong, Zhiqiang
- Keywords
- none
- MeSH Terms
-
- Animals
- Apoptosis/genetics*
- Caspases/genetics*
- Caspases/metabolism
- Fluorescence Resonance Energy Transfer*
- Genes, Reporter/genetics*
- Green Fluorescent Proteins/chemistry
- Green Fluorescent Proteins/genetics*
- Green Fluorescent Proteins/metabolism
- Humans
- Tumor Cells, Cultured
- Zebrafish
- PubMed
- 27447051 Full text @ Cell Chem Biol
Citation
To, T.L., Schepis, A., Ruiz-González, R., Zhang, Q., Yu, D., Dong, Z., Coughlin, S.R., Shu, X. (2016) Rational Design of a GFP-Based Fluorogenic Caspase Reporter for Imaging Apoptosis In Vivo. Cell chemical biology. 23:875-82.
Abstract
Fluorescence resonance energy transfer-based executioner caspase reporters using GFP are important tools for imaging apoptosis. While these reporters are useful for imaging apoptosis in cultured cells, their in vivo application has been handicapped by poor signal to noise. Here, we report the design and characterization of a GFP-based fluorogenic protease reporter, dubbed ZipGFP. ZipGFP-based TEV protease reporter increased fluorescence 10-fold after activation by protease. A ZipGFP-based executioner caspase reporter visualized apoptosis in live zebrafish embryos with spatiotemporal resolution. Thus, the ZipGFP-based caspase reporter may be useful for monitoring apoptosis during animal development and for designing reporters of proteases beyond the executioner caspases.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping