PUBLICATION
Traditional herbal prescription LASAP-C inhibits melanin synthesis in B16F10 melanoma cells and zebrafish
- Authors
- Kim, M.K., Bang, C.Y., Kim, M.Y., Lee, J.H., Ro, H., Choi, M.S., Kim, D.I., Jang, Y.P., Choung, S.Y.
- ID
- ZDB-PUB-160719-1
- Date
- 2016
- Source
- BMC complementary and alternative medicine 16: 223 (Journal)
- Registered Authors
- Kim, Dong-Il, Ro, Hyunju
- Keywords
- Herbal prescription, LASAP-C, Melanin, TRP-1, TRP-2, Tyrosinase, Zebrafish
- MeSH Terms
-
- Animals
- Antineoplastic Agents, Phytogenic/chemistry
- Antineoplastic Agents, Phytogenic/therapeutic use*
- Cell Line, Tumor
- Drug Screening Assays, Antitumor
- Intramolecular Oxidoreductases/metabolism
- Melanins/biosynthesis*
- Melanoma, Experimental/drug therapy*
- Melanoma, Experimental/metabolism
- Membrane Glycoproteins/metabolism
- Mice
- Mice, Inbred C57BL
- Monophenol Monooxygenase/metabolism
- Oxidoreductases/metabolism
- Pharmaceutical Preparations
- Phytotherapy*
- Pigmentation/drug effects
- Plant Extracts/therapeutic use*
- Zebrafish
- PubMed
- 27424198 Full text @ BMC Complement Altern Med
Citation
Kim, M.K., Bang, C.Y., Kim, M.Y., Lee, J.H., Ro, H., Choi, M.S., Kim, D.I., Jang, Y.P., Choung, S.Y. (2016) Traditional herbal prescription LASAP-C inhibits melanin synthesis in B16F10 melanoma cells and zebrafish. BMC complementary and alternative medicine. 16:223.
Abstract
Background In this study, the anti-melanogenesis efficacy of clinically used herbal prescription LASAP-C, which consists of four herbal medicines-Rehmanniae Radix Crudus, Lycii Fructus, Scutellariae Radix, and Angelicae Dahuricae Radix, was investigated.
Methods The chemical profile of LASAP-C was established by conducting ultra-performance liquid chromatography-electrospray ionization-mass spectrometry. Anti-melanogenic efficacy was evaluated by tyrosinase, tyrosinase-related protein (TRP)-1, and TRP-2 expression in B16F10 melanoma cells. In vivo evaluation was performed by using zebrafish model.
Results Molecular evidences suggested that melanin synthesis was inhibited via the down-regulation of tyrosinase, tyrosinase-related protein (TRP)-1, and TRP-2 expression in B16F10 melanoma cells treated with LASAP-C. The anti-melanogenesis efficacy was also confirmed in vivo by using the zebrafish model.
Conclusion The results of this study provide strong evidences that LASAP-C can be used as an active component in cosmeceutical products for reducing excess pigmentation in the human skin.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping