|ZFIN ID: ZDB-PUB-160719-1|
Traditional herbal prescription LASAP-C inhibits melanin synthesis in B16F10 melanoma cells and zebrafish
Kim, M.K., Bang, C.Y., Kim, M.Y., Lee, J.H., Ro, H., Choi, M.S., Kim, D.I., Jang, Y.P., Choung, S.Y.
|Source:||BMC complementary and alternative medicine 16: 223 (Journal)|
|Registered Authors:||Kim, Dong-Il, Ro, Hyunju|
|Keywords:||Herbal prescription, LASAP-C, Melanin, TRP-1, TRP-2, Tyrosinase, Zebrafish|
|PubMed:||27424198 Full text @ BMC Complement Altern Med|
Kim, M.K., Bang, C.Y., Kim, M.Y., Lee, J.H., Ro, H., Choi, M.S., Kim, D.I., Jang, Y.P., Choung, S.Y. (2016) Traditional herbal prescription LASAP-C inhibits melanin synthesis in B16F10 melanoma cells and zebrafish. BMC complementary and alternative medicine. 16:223.
Background In this study, the anti-melanogenesis efficacy of clinically used herbal prescription LASAP-C, which consists of four herbal medicines-Rehmanniae Radix Crudus, Lycii Fructus, Scutellariae Radix, and Angelicae Dahuricae Radix, was investigated.
Methods The chemical profile of LASAP-C was established by conducting ultra-performance liquid chromatography-electrospray ionization-mass spectrometry. Anti-melanogenic efficacy was evaluated by tyrosinase, tyrosinase-related protein (TRP)-1, and TRP-2 expression in B16F10 melanoma cells. In vivo evaluation was performed by using zebrafish model.
Results Molecular evidences suggested that melanin synthesis was inhibited via the down-regulation of tyrosinase, tyrosinase-related protein (TRP)-1, and TRP-2 expression in B16F10 melanoma cells treated with LASAP-C. The anti-melanogenesis efficacy was also confirmed in vivo by using the zebrafish model.
Conclusion The results of this study provide strong evidences that LASAP-C can be used as an active component in cosmeceutical products for reducing excess pigmentation in the human skin.
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