ZFIN ID: ZDB-PUB-160719-1
Traditional herbal prescription LASAP-C inhibits melanin synthesis in B16F10 melanoma cells and zebrafish
Kim, M.K., Bang, C.Y., Kim, M.Y., Lee, J.H., Ro, H., Choi, M.S., Kim, D.I., Jang, Y.P., Choung, S.Y.
Date: 2016
Source: BMC complementary and alternative medicine   16: 223 (Journal)
Registered Authors: Kim, Dong-Il, Ro, Hyunju
Keywords: Herbal prescription, LASAP-C, Melanin, TRP-1, TRP-2, Tyrosinase, Zebrafish
MeSH Terms:
  • Animals
  • Antineoplastic Agents, Phytogenic/chemistry
  • Antineoplastic Agents, Phytogenic/therapeutic use*
  • Cell Line, Tumor
  • Drug Screening Assays, Antitumor
  • Intramolecular Oxidoreductases/metabolism
  • Melanins/biosynthesis*
  • Melanoma, Experimental/drug therapy*
  • Melanoma, Experimental/metabolism
  • Membrane Glycoproteins/metabolism
  • Mice
  • Mice, Inbred C57BL
  • Monophenol Monooxygenase/metabolism
  • Oxidoreductases/metabolism
  • Pharmaceutical Preparations
  • Phytotherapy*
  • Pigmentation/drug effects
  • Plant Extracts/therapeutic use*
  • Zebrafish
PubMed: 27424198 Full text @ BMC Complement Altern Med
In this study, the anti-melanogenesis efficacy of clinically used herbal prescription LASAP-C, which consists of four herbal medicines-Rehmanniae Radix Crudus, Lycii Fructus, Scutellariae Radix, and Angelicae Dahuricae Radix, was investigated.
The chemical profile of LASAP-C was established by conducting ultra-performance liquid chromatography-electrospray ionization-mass spectrometry. Anti-melanogenic efficacy was evaluated by tyrosinase, tyrosinase-related protein (TRP)-1, and TRP-2 expression in B16F10 melanoma cells. In vivo evaluation was performed by using zebrafish model.
Molecular evidences suggested that melanin synthesis was inhibited via the down-regulation of tyrosinase, tyrosinase-related protein (TRP)-1, and TRP-2 expression in B16F10 melanoma cells treated with LASAP-C. The anti-melanogenesis efficacy was also confirmed in vivo by using the zebrafish model.
The results of this study provide strong evidences that LASAP-C can be used as an active component in cosmeceutical products for reducing excess pigmentation in the human skin.