ZFIN ID: ZDB-PUB-160715-7
BMP and retinoic acid regulate anterior-posterior patterning of the non-axial mesoderm across the dorsal-ventral axis
Naylor, R.W., Skvarca, L.B., Thisse, C., Thisse, B., Hukriede, N.A., Davidson, A.J.
Date: 2016
Source: Nature communications   7: 12197 (Journal)
Registered Authors: Davidson, Alan, Hukriede, Neil, Naylor, Richard, Thisse, Bernard, Thisse, Christine
Keywords: none
MeSH Terms:
  • Animals
  • Body Patterning/drug effects
  • Body Patterning/genetics*
  • Bone Morphogenetic Protein 2/genetics*
  • Bone Morphogenetic Proteins/genetics
  • Embryo, Nonmammalian
  • Kidney/embryology*
  • Mesoderm/embryology*
  • Retinoic Acid 4-Hydroxylase/genetics*
  • Tretinoin/metabolism*
  • Tretinoin/pharmacology
  • Zebrafish/embryology*
  • Zebrafish Proteins/genetics*
PubMed: 27406002 Full text @ Nat. Commun.
Despite the fundamental importance of patterning along the dorsal-ventral (DV) and anterior-posterior (AP) axes during embryogenesis, uncertainty exists in the orientation of these axes for the mesoderm. Here we examine the origin and formation of the zebrafish kidney, a ventrolateral mesoderm derivative, and show that AP patterning of the non-axial mesoderm occurs across the classic gastrula stage DV axis while DV patterning aligns along the animal-vegetal pole. We find that BMP signalling acts early to establish broad anterior and posterior territories in the non-axial mesoderm while retinoic acid (RA) functions later, but also across the classic DV axis. Our data support a model in which RA on the dorsal side of the embryo induces anterior kidney fates while posterior kidney progenitors are protected ventrally by the RA-catabolizing enzyme Cyp26a1. This work clarifies our understanding of vertebrate axis orientation and establishes a new paradigm for how the kidney and other mesodermal derivatives arise during embryogenesis.