PUBLICATION
Angiopellosis as an Alternative Mechanism of Cell Extravasation
- Authors
- Allen, T.A., Gracieux, D., Talib, M., Tokarz, D.A., Hensley, M.T., Cores, J., Vandergriff, A., Tang, J., de Andrade, J.B., Dinh, P.U., Yoder, J.A., Cheng, K.
- ID
- ZDB-PUB-160630-16
- Date
- 2017
- Source
- Stem cells (Dayton, Ohio) 35(1): 170-180 (Journal)
- Registered Authors
- Tokarz, Debra, Yoder, Jeffrey A.
- Keywords
- angiopellosis, diapedesis, extravasation, stem cell infusion, transmigration
- MeSH Terms
-
- Animals
- Blood Vessels/physiology*
- CD11 Antigens/metabolism
- Cell Aggregation
- Cell Membrane/metabolism
- Cell Shape
- Dogs
- Extravasation of Diagnostic and Therapeutic Materials/pathology*
- Female
- Humans
- Injections
- Intravital Microscopy
- Male
- Mesenchymal Stem Cells
- Microspheres
- Myocytes, Cardiac/cytology
- Polymers/chemistry
- Rats
- Time Factors
- Transendothelial and Transepithelial Migration
- Zebrafish/metabolism
- PubMed
- 27350343 Full text @ Stem Cells
Citation
Allen, T.A., Gracieux, D., Talib, M., Tokarz, D.A., Hensley, M.T., Cores, J., Vandergriff, A., Tang, J., de Andrade, J.B., Dinh, P.U., Yoder, J.A., Cheng, K. (2017) Angiopellosis as an Alternative Mechanism of Cell Extravasation. Stem cells (Dayton, Ohio). 35(1):170-180.
Abstract
Stem cells possess the ability to home in and travel to damaged tissue when injected intravenously. For the cells to exert their therapeutic effect, they must cross the blood vessel wall and enter the surrounding tissues. The mechanism of extravasation injected stem cells employ for exit has yet to be characterized. Using intravital microscopy and a transgenic zebrafish line Tg(fli1a:egpf) with GFP-expressing vasculature, we documented the detailed extravasation processes in vivo for injected stem cells in comparison to white blood cells (WBCs). While WBCs left the blood vessels by the standard diapedesis process, injected cardiac and mesenchymal stem cells underwent a distinct method of extravasation that was markedly different from diapedesis. Here, the vascular wall undergoes an extensive remodeling to allow the cell to exit the lumen, while the injected cell remains distinctively passive in activity. We termed this process Angio-pello-sis, which represents an alternative mechanism of cell extravasation to the prevailing theory of diapedesis. This article is protected by copyright. All rights reserved.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping