PUBLICATION

Zebrafish lines expressing UAS-driven red probes for monitoring cytoskeletal dynamics

Authors
Mizoguchi, T., Kawakami, K., Itoh, M.
ID
ZDB-PUB-160628-17
Date
2016
Source
Genesis (New York, N.Y. : 2000)   54(9): 483-9 (Journal)
Registered Authors
Itoh, Motoyuki, Kawakami, Koichi, Mizoguchi, Takamasa
Keywords
EB1, F-actin, GAL4-UAS system, Lifeact, live imaging, microtubule
MeSH Terms
  • Actin Cytoskeleton/metabolism*
  • Animals
  • Animals, Genetically Modified/genetics
  • Luminescent Proteins/genetics
  • Luminescent Proteins/metabolism*
  • Promoter Regions, Genetic*
  • Zebrafish/genetics*
PubMed
27342687 Full text @ Genesis
Abstract
Actin filaments and microtubules are principal components of the cytoskeleton that regulate the basic cellular phenomena underlying many fundamental cellular processes. Therefore, analyzing their dynamics in living cells is important for understanding cellular events more precisely. In this paper, we report two novel transgenic zebrafish lines expressing red fluorescent proteins tagged with Lifeact or EB1 that interact with actin filaments and microtubule plus ends, respectively, under the control of the GAL4-UAS system. Using these transgenic lines, we could detect F-actin and microtubule plus end dynamics in specific tissues of living zebrafish embryos by crossing with GAL4 driver lines. In addition, we could achieve multi-color imaging using these transgenic lines with GFP-expressing transgenic lines. Therefore, our transgenic lines that carry UAS-driven red fluorescent cytoskeletal probes are useful tools for analyzing spatiotemporal changes of the cytoskeletal elements using multi-color live imaging.
Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes