PUBLICATION

Translocation of nanoparticles and Mycobacterium marinum across the intestinal epithelium in zebrafish and the role of the mucosal immune system

Authors
Løvmo, S.D., Speth, M.T., Repnik, U., Koppang, E.O., Griffiths, G.W., Hildahl, J.P.
ID
ZDB-PUB-160628-11
Date
2017
Source
Developmental and comparative immunology   67: 508-518 (Journal)
Registered Authors
Hildahl, Jon
Keywords
Antigen sampling, Intestine, Mucosal immunity, Mycobacterium marinum, Nanoparticles, Zebrafish
MeSH Terms
  • Biological Transport
  • Bacterial Translocation/immunology
  • Zebrafish/immunology*
  • Intestinal Mucosa/microbiology
  • Intestinal Mucosa/physiology*
  • Immunity, Mucosal
  • Antigen Presentation
  • Mycobacterium marinum/physiology*
  • Enterocytes/immunology*
  • Fish Diseases/immunology*
  • Nanoparticles/metabolism*
  • Drug Delivery Systems
  • Cells, Cultured
  • Mycobacterium Infections, Nontuberculous/immunology*
  • Transendothelial and Transepithelial Migration
  • Animals
(all 16)
PubMed
27343826 Full text @ Dev. Comp. Immunol.
Abstract
Nano- and microparticles are promising carrier systems for oral delivery of drugs or vaccines, particularly in fish aquaculture. However, the mechanisms of uptake, trans-epithelial transport and immune response to nano/micrometer sized particles, or microorganisms such as bacteria are poorly understood in fish. Here, adult zebrafish were used to study the uptake of different nano- and microparticles and the pathogenic bacteria Mycobacterium marinum in the intestine, and their interactions with epithelial cells and the mucosal immune system. Fluorescent particles or bacteria were delivered directly into the adult zebrafish intestine by oral intubation and their localization was imaged in intestine, liver and spleen sections. Zebrafish do not appear to have M-cells, but both nanoparticles and bacteria were rapidly taken up in the intestine and transported to the liver and spleen. In each tissue, both bacteria and particles largely localized to leukocytes, presumably macrophages.
Genes / Markers
Figures
No images available
Expression
Phenotype
No data available
Mutations / Transgenics
No data available
Human Disease / Model
No data available
Sequence Targeting Reagents
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Fish
No data available
Antibodies
Orthology
Engineered Foreign Genes
No data available
Mapping