PUBLICATION
Silver_ nanoparticles inhibited erythrogenesis during zebrafish embryogenesis
- Authors
- Cui, B., Ren, L., Xu, Q.H., Yin, L.Y., Zhou, X.Y., Liu, J.X.
- ID
- ZDB-PUB-160625-3
- Date
- 2016
- Source
- Aquatic toxicology (Amsterdam, Netherlands) 177: 295-305 (Journal)
- Registered Authors
- Liu, Jing-xia
- Keywords
- AgNPs, Erythrogenesis, Hemoglobin, Microarray, Zebrafish
- MeSH Terms
-
- Animals
- Down-Regulation/drug effects
- Embryo, Nonmammalian/drug effects
- Embryo, Nonmammalian/physiology
- Embryonic Development/drug effects*
- Embryonic Development/physiology
- Erythropoiesis/drug effects*
- Erythropoiesis/genetics
- Female
- Genetic Markers
- Hemoglobins/genetics
- Male
- Metal Nanoparticles/toxicity*
- Oligonucleotide Array Sequence Analysis
- Reverse Transcriptase Polymerase Chain Reaction
- Silver/toxicity*
- Water Pollutants, Chemical/toxicity*
- Zebrafish
- PubMed
- 27340786 Full text @ Aquat. Toxicol.
Citation
Cui, B., Ren, L., Xu, Q.H., Yin, L.Y., Zhou, X.Y., Liu, J.X. (2016) Silver_ nanoparticles inhibited erythrogenesis during zebrafish embryogenesis. Aquatic toxicology (Amsterdam, Netherlands). 177:295-305.
Abstract
Silver_ nanoparticles (AgNPs), for their attractive antimicrobial properties, have become one of the most commercial nanomaterials used recently. AgNPs are reported to be toxic to blood cells of aquatic organisms and humans, however, few studies related to toxic effects of AgNPs in hematopoiesis using an in vivo model were available. Firstly, microarrays were applied to reveal transcriptional responses of zebrafish embryos to AgNPs at 24h post-fertilization (hpf)in this study, and hemoglobin genes were found to be down-regulated by AgNPs and to be enriched in the top 10 categories by Gene Ontology (GO) analysis. The reduced expressions of hemoglobin were further demonstrated by qRT-PCR detection, whole-mount in situ hybridization, and O-dianisidine staining at transcriptional and translational level. Next, the commitment of mesoderm, specification of hematopoietic progenitor cells and differentiation of erythroids were detected at different developmental stages in AgNPs-exposed embryos, and erythrogenesis were found to be inhibited by AgNPs in developmental-stage-specific and cell-specific manners. Finally, it was pointed out that AgNPs affected erythrogenesis mostly by their particles other than their releasing ions.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping