Imaging collective cell migration and hair cell regeneration in the sensory lateral line
- Venero Galanternik, M., Navajas Acedo, J., Romero-Carvajal, A., Piotrowski, T.
- Methods in cell biology 134: 211-56 (Chapter)
- Registered Authors
- Carvajal, Andres Romero, Navajas Acedo, Joaquin, Piotrowski, Tatjana, Venero Galanternik, Marina
- Collective migration, Confocal imaging, Hair cells, Primordium, Regeneration, Sensory system, Spatial analysis, Time lapse, Transgenic lines, Zebrafish embryo
- MeSH Terms
- Animals, Genetically Modified
- Cell Differentiation/genetics*
- Cell Movement/genetics
- Cell Proliferation/genetics
- Cell Shape
- Hair Cells, Auditory/ultrastructure*
- Lateral Line System/growth & development
- Lateral Line System/ultrastructure*
- Microscopy, Confocal/methods*
- 27312495 Full text @ Meth. Cell. Biol.
Venero Galanternik, M., Navajas Acedo, J., Romero-Carvajal, A., Piotrowski, T. (2016) Imaging collective cell migration and hair cell regeneration in the sensory lateral line. Methods in cell biology. 134:211-56.
The accessibility of the lateral line system and its amenability to long-term in vivo imaging transformed the developing lateral line into a powerful model system to study fundamental morphogenetic events, such as guided migration, proliferation, cell shape changes, organ formation, organ deposition, cell specification and differentiation. In addition, the lateral line is not only amenable to live imaging during migration stages but also during postembryonic events such as sensory organ tissue homeostasis and regeneration. The robust regenerative capabilities of the mature, mechanosensory lateral line hair cells, which are homologous to inner ear hair cells and the ease with which they can be imaged, have brought zebrafish into the spotlight as a model to develop tools to treat human deafness. In this chapter, we describe protocols for long-term in vivo confocal imaging of the developing and regenerating lateral line.
Genes / Markers
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Engineered Foreign Genes
Errata and Notes