PUBLICATION
A Novel Long-term, Multi-Channel and Non-invasive Electrophysiology Platform for Zebrafish
- Authors
- Hong, S., Lee, P., Baraban, S.C., Lee, L.P.
- ID
- ZDB-PUB-160617-6
- Date
- 2016
- Source
- Scientific Reports 6: 28248 (Journal)
- Registered Authors
- Baraban, Scott
- Keywords
- Electroencephalography EEG, High-throughput screening, Lab-on-a-chip
- MeSH Terms
-
- Microelectrodes
- Epilepsy/drug therapy
- Epilepsy/physiopathology
- Electrophysiological Phenomena
- Microfluidics
- Animals
- Anticonvulsants/pharmacology
- Anticonvulsants/therapeutic use
- Zebrafish/physiology*
- PubMed
- 27305978 Full text @ Sci. Rep.
Citation
Hong, S., Lee, P., Baraban, S.C., Lee, L.P. (2016) A Novel Long-term, Multi-Channel and Non-invasive Electrophysiology Platform for Zebrafish. Scientific Reports. 6:28248.
Abstract
Zebrafish are a popular vertebrate model for human neurological disorders and drug discovery. Although fecundity, breeding convenience, genetic homology and optical transparency have been key advantages, laborious and invasive procedures are required for electrophysiological studies. Using an electrode-integrated microfluidic system, here we demonstrate a novel multichannel electrophysiology unit to record multiple zebrafish. This platform allows spontaneous alignment of zebrafish and maintains, over days, close contact between head and multiple surface electrodes, enabling non-invasive long-term electroencephalographic recording. First, we demonstrate that electrographic seizure events, induced by pentylenetetrazole, can be reliably distinguished from eye or tail movement artifacts, and quantifiably identified with our unique algorithm. Second, we show long-term monitoring during epileptogenic progression in a scn1lab mutant recapitulating human Dravet syndrome. Third, we provide an example of cross-over pharmacology antiepileptic drug testing. Such promising features of this integrated microfluidic platform will greatly facilitate high-throughput drug screening and electrophysiological characterization of epileptic zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping