Three-dimensional spatiotemporal focusing of holographic patterns
- Hernandez, O., Papagiakoumou, E., Tanese, D., Fidelin, K., Wyart, C., Emiliani, V.
- Nature communications 7: 11928 (Journal)
- Registered Authors
- Wyart, Claire
- Physical sciences, Optical physics
- MeSH Terms
- Image Processing, Computer-Assisted/methods
- Imaging, Three-Dimensional/methods*
- Larva/growth & development
- Microscopy, Phase-Contrast/instrumentation
- Microscopy, Phase-Contrast/methods*
- Optical Devices*
- Zebrafish/growth & development
- 27306044 Full text @ Nat. Commun.
Hernandez, O., Papagiakoumou, E., Tanese, D., Fidelin, K., Wyart, C., Emiliani, V. (2016) Three-dimensional spatiotemporal focusing of holographic patterns. Nature communications. 7:11928.
Two-photon excitation with temporally focused pulses can be combined with phase-modulation approaches, such as computer-generated holography and generalized phase contrast, to efficiently distribute light into two-dimensional, axially confined, user-defined shapes. Adding lens-phase modulations to 2D-phase holograms enables remote axial pattern displacement as well as simultaneous pattern generation in multiple distinct planes. However, the axial confinement linearly degrades with lateral shape area in previous reports where axially shifted holographic shapes were not temporally focused. Here we report an optical system using two spatial light modulators to independently control transverse- and axial-target light distribution. This approach enables simultaneous axial translation of single or multiple spatiotemporally focused patterns across the sample volume while achieving the axial confinement of temporal focusing. We use the system's capability to photoconvert tens of Kaede-expressing neurons with single-cell resolution in live zebrafish larvae.
Genes / Markers
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Engineered Foreign Genes
Errata and NotesThis article is corrected by ZDB-PUB-220906-52.