PUBLICATION
Microarray Noninvasive Neuronal Seizure Recordings from Intact Larval Zebrafish
- Authors
- Meyer, M., Dhamne, S.C., LaCoursiere, C.M., Tambunan, D., Poduri, A., Rotenberg, A.
- ID
- ZDB-PUB-160610-1
- Date
- 2016
- Source
- PLoS One 11: e0156498 (Journal)
- Registered Authors
- Keywords
- Action potentials, Larvae, Zebrafish, Epilepsy, Electrode recording, Electroencephalography, Brain electrophysiology, Epileptic seizures
- MeSH Terms
-
- Action Potentials
- Animals
- Brain/drug effects
- Brain/pathology*
- Electroencephalography
- Electrophysiological Phenomena
- Epilepsy/chemically induced
- Epilepsy/physiopathology*
- Larva/drug effects
- Larva/physiology*
- Microelectrodes
- Neurons/drug effects
- Neurons/pathology*
- Pentylenetetrazole/toxicity
- Seizures/chemically induced
- Seizures/physiopathology*
- Zebrafish/physiology*
- PubMed
- 27281339 Full text @ PLoS One
Citation
Meyer, M., Dhamne, S.C., LaCoursiere, C.M., Tambunan, D., Poduri, A., Rotenberg, A. (2016) Microarray Noninvasive Neuronal Seizure Recordings from Intact Larval Zebrafish. PLoS One. 11:e0156498.
Abstract
Zebrafish epilepsy models are emerging tools in experimental epilepsy. Zebrafish larvae, in particular, are advantageous because they can be easily genetically altered and used for developmental and drug studies since agents applied to the bath penetrate the organism easily. Methods for electrophysiological recordings in zebrafish are new and evolving. We present a novel multi-electrode array method to non-invasively record electrical activity from up to 61 locations of an intact larval zebrafish head. This method enables transcranial noninvasive recording of extracellular field potentials (which include multi-unit activity and EEG) to identify epileptic seizures. To record from the brains of zebrafish larvae, the dorsum of the head of an intact larva was secured onto a multi-electrode array. We recorded from individual electrodes for at least three hours and quantified neuronal firing frequency, spike patterns (continuous or bursting), and synchrony of neuronal firing. Following 15 mM potassium chloride- or pentylenetetrazole-infusion into the bath, spike and burst rate increased significantly. Additionally, synchrony of neuronal firing across channels, a hallmark of epileptic seizures, also increased. Notably, the fish survived the experiment. This non-invasive method complements present invasive zebrafish neurophysiological techniques: it affords the advantages of high spatial and temporal resolution, a capacity to measure multiregional activity and neuronal synchrony in seizures, and fish survival for future experiments, such as studies of epileptogenesis and development.
Errata / Notes
Correction.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping