ZFIN ID: ZDB-PUB-160608-19
Cellular dissection of zebrafish hematopoiesis
Stachura, D.L., Traver, D.
Date: 2016
Source: Methods in cell biology   133: 11-53 (Chapter)
Registered Authors: Traver, David
Keywords: Hematopoietic cell enrichment and transplantation, Zebrafish adult and developmental hematopoiesis, Zebrafish clonal hematopoietic cell assays, Zebrafish in vitro cell culture
MeSH Terms:
  • Animals
  • Cell Culture Techniques/methods*
  • Cell Differentiation
  • Cell Separation
  • Disease Models, Animal
  • Embryo, Nonmammalian/metabolism
  • Hematopoiesis*
  • Hematopoietic Stem Cells/cytology*
  • Zebrafish/growth & development
  • Zebrafish/physiology*
PubMed: 27263407 Full text @ Meth. Cell. Biol.
Zebrafish as a model system have been instrumental in understanding early vertebrate development, especially of the hematopoietic system. The external development of zebrafish and their genetic amenability have allowed in-depth studies of multiple blood cell types and their respective genetic regulation. This chapter highlights some new data in zebrafish hematopoiesis regarding primitive and definitive hematopoiesis in the embryonic and adult fish, allowing the isolation of prospective progenitor subsets. It also highlights assays developed to examine the function of these progenitors in vivo and in vitro, allowing an evolutionary understanding of the hematopoietic system and how zebrafish can be better utilized as a model system for a multitude of hematopoietic disorders.