PUBLICATION

Cellular dissection of zebrafish hematopoiesis

Authors
Stachura, D.L., Traver, D.
ID
ZDB-PUB-160608-19
Date
2016
Source
Methods in cell biology   133: 11-53 (Chapter)
Registered Authors
Traver, David
Keywords
Hematopoietic cell enrichment and transplantation, Zebrafish adult and developmental hematopoiesis, Zebrafish clonal hematopoietic cell assays, Zebrafish in vitro cell culture
MeSH Terms
  • Animals
  • Cell Culture Techniques/methods*
  • Cell Differentiation
  • Cell Separation
  • Disease Models, Animal
  • Embryo, Nonmammalian/metabolism
  • Hematopoiesis*
  • Hematopoietic Stem Cells/cytology*
  • Zebrafish/growth & development
  • Zebrafish/physiology*
PubMed
27263407 Full text @ Meth. Cell. Biol.
Abstract
Zebrafish as a model system have been instrumental in understanding early vertebrate development, especially of the hematopoietic system. The external development of zebrafish and their genetic amenability have allowed in-depth studies of multiple blood cell types and their respective genetic regulation. This chapter highlights some new data in zebrafish hematopoiesis regarding primitive and definitive hematopoiesis in the embryonic and adult fish, allowing the isolation of prospective progenitor subsets. It also highlights assays developed to examine the function of these progenitors in vivo and in vitro, allowing an evolutionary understanding of the hematopoietic system and how zebrafish can be better utilized as a model system for a multitude of hematopoietic disorders.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping