PUBLICATION

Engineering Hematopoietic Stem Cells: Lessons from Development

Authors
Rowe, R.G., Mandelbaum, J., Zon, L.I., Daley, G.Q.
ID
ZDB-PUB-160604-5
Date
2016
Source
Cell Stem Cell   18: 707-720 (Review)
Registered Authors
Zon, Leonard I.
Keywords
none
MeSH Terms
  • Animals
  • Cell Engineering/methods*
  • Embryonic Development*
  • Hematopoiesis
  • Hematopoietic Stem Cells/cytology*
  • Humans
  • Models, Biological
  • Time Factors
PubMed
27257760 Full text @ Cell Stem Cell
Abstract
Cell engineering has brought us tantalizingly close to the goal of deriving patient-specific hematopoietic stem cells (HSCs). While directed differentiation and transcription factor-mediated conversion strategies have generated progenitor cells with multilineage potential, to date, therapy-grade engineered HSCs remain elusive due to insufficient long-term self-renewal and inadequate differentiated progeny functionality. A cross-species approach involving zebrafish and mammalian systems offers complementary methodologies to improve understanding of native HSCs. Here, we discuss the role of conserved developmental timing processes in vertebrate hematopoiesis, highlighting how identification and manipulation of stage-specific factors that specify HSC developmental state must be harnessed to engineer HSCs for therapy.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping