PUBLICATION
Protein palmitoylation activate zygotic gene expression during the maternal-to-zygotic transition
- Authors
- Du, Z., Chen, X., Li, X., He, K., Ji, S., Shi, W., Hao, A.
- ID
- ZDB-PUB-160529-4
- Date
- 2016
- Source
- Biochemical and Biophysical Research Communications 475(2): 194-201 (Journal)
- Registered Authors
- Keywords
- Mid-Blastula Transition (MBT), Palmitoylation, Zebrafish, miR-430
- MeSH Terms
-
- Animals
- DNA Methylation
- Female
- Gene Expression Regulation, Developmental*
- Lipoylation*
- MicroRNAs/genetics*
- Zebrafish/embryology*
- Zebrafish/genetics
- Zebrafish/metabolism
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- Zygote/growth & development
- Zygote/metabolism*
- PubMed
- 27235108 Full text @ Biochem. Biophys. Res. Commun.
Citation
Du, Z., Chen, X., Li, X., He, K., Ji, S., Shi, W., Hao, A. (2016) Protein palmitoylation activate zygotic gene expression during the maternal-to-zygotic transition. Biochemical and Biophysical Research Communications. 475(2):194-201.
Abstract
Upon fertilization, maternal factors direct development and trigger zygotic genome activation at the maternal-to-zygotic transition (MZT). However, the factors that activate the zygotic program in vertebrates are not well defined. Here, we found that protein palmitoylation played an important role in acquiring transcriptional competency and orchestrating the clearance of the maternal program in zebrafish. After inhibition of protein palmitoylation, zebrafish embryos developed normally before the Mid-Blastula Transition (MBT); however, they did not initiate epiboly. Moreover, our results showed that protein palmitoylation is required to initiate the zygotic developmental program and induce clearance of the maternal program by activating miR-430 expression.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping