PUBLICATION

RNA polymerase III component Rpc9 regulates hematopoietic stem and progenitor cell maintenance in zebrafish

Authors
Wei, Y., Xu, J., Zhang, W., Wen, Z., Liu, F.
ID
ZDB-PUB-160507-8
Date
2016
Source
Development (Cambridge, England)   143(12): 2103-10 (Journal)
Registered Authors
Liu, Feng, Wei, Yonglong, Wen, Zilong
Keywords
RNA polymerase III, Rpc9, P53, Hematopoietic stem and progenitor cells, Zebrafish
MeSH Terms
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cell Proliferation
  • Cell Survival
  • Embryo, Nonmammalian/metabolism
  • Gene Knockdown Techniques
  • Hematopoiesis
  • Hematopoietic Stem Cells/cytology*
  • Hematopoietic Stem Cells/metabolism*
  • Mutation/genetics
  • Phenotype
  • RNA Polymerase III/metabolism*
  • T-Lymphocytes/cytology
  • Tumor Suppressor Protein p53/metabolism
  • Zebrafish/metabolism*
  • Zebrafish Proteins/metabolism*
PubMed
27151951 Full text @ Development
Abstract
Hematopoietic stem and progenitor cells (HSPCs) are capable of self-renewal and replenishing all lineages of blood cells throughout the lifetime and thus critical for tissue homeostasis. However, the mechanism regulating HSPC development is still incompletely understood. Here, we isolate a zebrafish mutant with defective T lymphopoiesis and positional cloning identifies that Rpc9, a component of DNA-directed RNA polymerase III (Pol III) complex, is responsible for the mutant phenotype. Further analysis shows that rpc9-deficiency leads to the impairment of HSPCs and their derivatives in zebrafish embryos. Excessive apoptosis is observed in the caudal hematopoietic tissue (CHT, the equivalent of fetal liver in mammals) of rpc9(-/-) embryos and the hematopoietic defects in rpc9(-/-) embryos can be fully rescued by suppression of p53 Thus, our work illustrate that Rpc9, a component of Pol III, plays an important tissue-specific role in HSPC maintenance during zebrafish embryogenesis and that it might be conserved across vertebrates including mammals.
Genes / Markers
Figures
Figure Gallery
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Errata and Notes