ZFIN ID: ZDB-PUB-160501-3
MicroRNA-140-5p impairs zebrafish embryonic bone development via targeting BMP-2
Gan, S., Huang, Z., Liu, N., Su, R., Xie, G., Zhong, B., Zhang, K., Wang, S., Hu, X., Zhang, J., Xiang, S.
Date: 2016
Source: FEBS letters   590(10): 1438-46 (Journal)
Registered Authors: Zhang, Jian
Keywords: BMP-2, MicroRNA-140-5p, bone and cartilage development, embryos, zebrafish
MeSH Terms:
  • 3' Untranslated Regions
  • Animals
  • Bone Development*
  • Bone Morphogenetic Protein 2/genetics*
  • Conserved Sequence
  • Embryonic Development
  • Gene Expression Regulation, Developmental
  • Humans
  • MicroRNAs/genetics*
  • Vertebrates/genetics
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Zebrafish Proteins/genetics*
PubMed: 27130837 Full text @ FEBS Lett.
MicroRNA-140-5p (miRNA-140-5p) is important for embryonic bone development. In the present study, we found that miRNA-140-5p and its binding site in the 3'UTR of bone morphogenetic protein 2 (BMP-2) are highly conserved among vertebrates, and miRNA-140-5p negatively regulates both zebrafish and human BMP-2 genes. Microinjection of miRNA-140-5p or BMP-2b morpholino into zebrafish embryos led to a similar phenotype, including shortened tails, curved trunks and defects in cranial cartilage. Moreover, miRNA-140-5p injection induced zebrafish embryo malformation that could be significantly rescued by microinjection of BMP-2 mRNA. In conclusion, our results indicated that miRNA-140-5p regulates zebrafish embryonic bone development via targeting BMP-2. This article is protected by copyright. All rights reserved.