PUBLICATION
Ihha induces hybrid cartilage-bone cells during zebrafish jawbone regeneration
- Authors
- Paul, S., Schindler, S., Giovannone, D., de Millo Terrazzani, A., Mariani, F.V., Crump, J.G.
- ID
- ZDB-PUB-160429-9
- Date
- 2016
- Source
- Development (Cambridge, England) 143(12): 2066-76 (Journal)
- Registered Authors
- Crump, Gage DeKoeyer, Schindler, Simone
- Keywords
- Bone regeneration, Zebrafish, Jaw, Chondrocyte, Osteoblast, Ihha, Chondroid bone
- MeSH Terms
-
- Aging/physiology
- Animals
- Biomarkers/metabolism
- Bone Regeneration*
- Calcification, Physiologic/genetics
- Cartilage/cytology*
- Cell Lineage
- Chondrocytes/cytology
- Gene Expression Regulation, Developmental
- Growth Plate/embryology
- Hedgehog Proteins/metabolism*
- Jaw/embryology
- Jaw/physiology*
- Models, Biological
- Osteoblasts/cytology*
- Periosteum/cytology
- Wound Healing
- Zebrafish/embryology
- Zebrafish/genetics
- Zebrafish/physiology*
- Zebrafish Proteins/metabolism*
- PubMed
- 27122168 Full text @ Development
Citation
Paul, S., Schindler, S., Giovannone, D., de Millo Terrazzani, A., Mariani, F.V., Crump, J.G. (2016) Ihha induces hybrid cartilage-bone cells during zebrafish jawbone regeneration. Development (Cambridge, England). 143(12):2066-76.
Abstract
The healing of bone often involves a cartilage intermediate, yet how such cartilage is induced and utilized during repair is not fully understood. By studying a model of large-scale bone regeneration in the lower jaw of adult zebrafish, we show that chondrocytes are critical for generating thick bone during repair. During jawbone regeneration, we find that chondrocytes co-express genes associated with osteoblast differentiation and produce extensive mineralization, which is in marked contrast to chondrocytes during facial skeletal development. We also identify the likely source of repair chondrocytes as a population of Runx2+, Sp7- cells that emanate from the periosteum, a tissue that normally contributes only osteoblasts during homeostasis. Analysis of ihha mutants shows that the ability of periosteal cells to generate cartilage in response to injury depends on a repair-specific role of Ihha in the induction as opposed to the proliferation of chondrocytes. The large-scale regeneration of the zebrafish jawbone thus employs a cartilage differentiation program distinct from that seen during development, with the bone-forming potential of repair chondrocytes potentially due to their derivation from osteogenic cells in the periosteum.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping